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11207733910

Roche

抗-地高辛-POD，Fab片段

from sheep

别名:

地高辛配基, 抗-地高辛配基

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About This Item

UNSPSC代码:
12352203
价格与库存信息目前不能提供

生物来源

sheep

质量水平

100

偶联物

peroxidase conjugate

抗体形式

F(ab′)2 fragment of affinity isolated antibody

抗体产品类型

primary antibodies

克隆

polyclonal

表单

lyophilized (clear, brown solution after reconstitution)

包装

pkg of 150 U

制造商/商品名称

Roche

储存温度

2-8°C

相关类别

一般描述

地高辛是一种半抗原，可用于核酸标记和检测系统。[1]本产品含辣根过氧化物酶（POD）标记抗地高辛抗体的Fab片段。抗地高辛-POD Fab片段用于检测地高辛标记的化合物。

特异性

来自羊的多克隆抗体对于地高辛和异羟洋地黄毒苷有特异性，不会和其他类固醇，比如人雌激素和雄激素反应。

应用

使用抗-地高辛-POD，Fab片段用来在以下实验中检测地高辛-标记化合物:
  • 斑点印迹法
  • ELISA
  • 免疫组织化学[2]
  • 原位杂交[3][4]
  • Western印迹法[5]

制备说明

在用地高辛免疫后，山羊IgG通过离心交换色谱进行了纯化，而特异性的IgG通过免疫吸附进行了分离。通过木瓜蛋白酶消化获得的Fab片段经过凝胶过滤，和特异性标记偶联，在缓冲液中稳定。

工作浓度: 偶联剂的工作浓度将取决于应用和底物。以下浓度可作为参考：斑点印迹：150 mU/ml
  • ELISA: 50至150 mU/ml
  • 免疫组织化学：250至500 mU/ml
  • 原位杂交：1.5至7.5 mU/ml
  • Southern blot：150 mU/ml
  • Western blot：250至500 mU/ml

工作溶液：100mM Tris-HCl,150mM NaCl,pH 7.5.
1% 阻断试剂(w/v), 1到5%热失活胎牛血清(v/v)或羊正常血清，可以用来降低非特异性结合。

重悬

加入1ml双蒸馏水，最终浓度为150 U/ml。

其他说明

仅用于生命科学研究。不可用于诊断。

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象形图

Exclamation mark
GHS07

警示用语：

Warning

危险声明

H317

危险分类

Skin Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

does not flash

闪点(°C)

does not flash

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
74946000
66842300
64012000
43500600
28557000

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Hande Tunbak et al.
eLife, 9 (2020-05-06)
The zebrafish was used to assess the impact of social isolation on behaviour and brain function. As in humans and other social species, early social deprivation reduced social preference in juvenile zebrafish. Whole-brain functional maps of anti-social isolated (lonely) fish
X Peng et al.
Leukemia, 29(12), 2355-2365 (2015-06-25)
Controlled self-renewal and differentiation of hematopoietic stem/progenitor cells (HSPCs) are critical for vertebrate development and survival. These processes are tightly regulated by the transcription factors, signaling molecules and epigenetic factors. Impaired regulations of their function could result in hematological malignancies.
Sarah E Mercer et al.
PloS one, 7(12), e52375-e52375 (2013-01-10)
The inability to functionally repair tissues that are lost as a consequence of disease or injury remains a significant challenge for regenerative medicine. The molecular and cellular processes involved in complete restoration of tissue architecture and function are expected to
Yutaka Daido et al.
Developmental biology, 392(1), 117-129 (2014-05-07)
The vertebrate retina contains two types of photoreceptor cells, rods and cones, which use distinct types of opsins and phototransduction proteins. Cones can be further divided into several subtypes with differing wavelength sensitivity and morphology. Although photoreceptor development has been
Juha Kotimaa et al.
Molecular immunology, 76, 13-21 (2016-06-24)
Experimental mouse models have been extensively used to elucidate the role of the complement system in different diseases and injuries. Contribution of gender has revealed an intriguing gender specific difference; female mice often show protection against most complement driven injuries
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