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11685619910

Roche

荧光素RNA标记混合物

solution, suitable for Northern blotting, suitable for Southern blotting, suitable for hybridization

别名:

RNA标记

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About This Item

分類程式碼代碼:
41105500

形狀

solution

品質等級

100

用途

sufficient for 20 reactions

包裝

pkg of 40 μL

製造商／商標名

Roche

技術

Northern blotting: suitable
Southern blotting: suitable
hybridization: suitable

儲存溫度

−20°C

相关类别

一般說明

荧光素RNA标记混合物是使用便捷的核苷酸混合物，用于用荧光素-12-尿苷三磷酸（UTP）标记RNA。

特異性

热灭活：添加2μl 0.2M EDTA (pH 8.0) 终止反应。

應用

荧光素标记的RNA用于下列多种杂交技术：
  • 组织原位杂交技术
  • Southern 印迹
  • Northern 印迹
  • 菌斑或菌落转移
  • 核糖核酸酶保护实验

品質

该混合物的功能是通过与DIG RNA标记试剂盒结合进行测定的。

原則

通过体外转录产生具有确定长度的荧光素标记的单链RNA探针。在标准条件下，SP6、T7和T3 RNA聚合酶可在转录物的大约每20至25个核苷酸处掺入荧光素-12-UTP。荧光素RNA标记混合物专门设计用于RNA聚合酶SP6、T7和T3，并且都配有优化的转录缓冲液。

準備報告

10x浓缩NTP标记混合物：10mM ATP，10mM CTP，10mM GTP，6.5mM UTP，3.5mM荧光素-12-UTP，pH 7.5（+20℃）。

其他說明

仅用于生命科学研究。不可用于诊断。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

nwg

閃點（°F）

No data available

閃點（°C）

No data available

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
59973820
41027920
32874620
24158720
70036320

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