콘텐츠로 건너뛰기
Merck
모든 사진(3)

주요 문서

MAK078

Sigma-Aldrich

Xanthine Oxidase Activity Assay Kit

sufficient for 100 colorimetric or fluorometric tests

동의어(들):

XO Activity Assay Kit

로그인조직 및 계약 가격 보기
모든 사진(3)

About This Item

UNSPSC 코드:
12161503
NACRES:
NA.84

사용

sufficient for 100 colorimetric or fluorometric tests

검출 방법

colorimetric
fluorometric

관련 질환(들)

rheumatological diseases; gastrointestinal diseases; cardiovascular diseases

저장 온도

−20°C

유전자 정보

human ... XDH(7498)
mouse ... XDH(22436)
rat ... XDH(497811)

일반 설명

Xanthine Oxidase (XO) catalyzes the sequential oxidation of hypoxanthine to xanthine, and xanthine to uric acid and hydrogen peroxide. In humans and other primates, XO controls the final step of purine catabolism and is normally found in the liver and the intestinal mucosa. In rodents, XO is broadly expressed in most tissues. While XO activity is normally very low in blood, liver injury can result in the release of XO into blood. XO may contribute to the pathogenesis of gout and cardiovascular disease, and XO activity or expression may be upregulated in these conditions.

애플리케이션

Xanthine Oxidase Activity Assay Kit has been used to measure the xanthine oxidase in serum samples.[1][2]

적합성

Suitable for the measurement of xanthine oxidase in a variety of samples including tissue, cells and serum

원리

The Xanthine Oxidase Activity Assay Kit provides a simple and direct procedure for measuring XO activity in a variety of biological samples. XO activity is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the hydrogen peroxide generated. One unit of XO is defined as the amount of enzyme that catalyzes the oxidation of xanthine, yielding 1.0 μmole of uric acid and hydrogen peroxide per minute at 25 °C.

교체됨

제품 번호
설명
가격

Storage Class Code

10 - Combustible liquids

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
8J12K07100
8F10K07100
8D01K07100
7H27K07100
7G02K07100

적합한 버전을 찾을 수 없으신가요?

특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

이미 열람한 고객

Slide 1 of 2

1 of 2

Xanthine BioUltra, ≥99%

Sigma-Aldrich

X4002

Xanthine

Catalase Assay Kit sufficient for ≥100 tests enzymatic, determination of catalase activity in tissues and cells

Sigma-Aldrich

CAT100

Catalase Assay Kit

Probiotic therapy alleviates hyperuricemia in C57BL/6 mouse model.
Cao T, et al.
Biomedical Research, 28(5) (2017)
Shivangi Rastogi et al.
PLoS pathogens, 17(7), e1009712-e1009712 (2021-07-30)
Mycobacterium tuberculosis (Mtb) has evolved to evade host innate immunity by interfering with macrophage functions. Interleukin-1β (IL-1β) is secreted by macrophages after the activation of the inflammasome complex and is crucial for host defense against Mtb infections. We have previously
Anti-gouty arthritis and antihyperuricemia effects of sunflower (Helianthus annuus) head extract in gouty and hyperuricemia animal models.
Li L, et al.
BioMed Research International, 2017, 9-9 (2017)
Neelam Malik et al.
BMC chemistry, 13(1), 71-71 (2019-08-07)
Xanthine oxidase is an important enzyme of purine catabolism pathway and has been associated directly in pathogenesis of gout and indirectly in many pathological conditions like cancer, diabetes and metabolic syndrome. In this research rutin, a bioactive flavonoid was explored
Ha Thi Nguyen et al.
PloS one, 16(6), e0253572-e0253572 (2021-07-01)
In the present study, in silico predictions and molecular docking were performed on five clerodane diterpenes (1-5) from Polyalthia longifolia seeds to evaluate their potential as xanthine oxidase (XO) inhibitors. The initial screening was conducted by target prediction using TargetNet
모든 관련된 서류 보기

질문

1–3 / 3 질문  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 답변

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      도움이 되었습니까?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 답변

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      도움이 되었습니까?

  3. Does the kit allow the use of cell pellets that have been frozen at -80°C, or do the samples need to be fresh, considering that the cells should be rapidly homogenized with the assay buffer?

    1 답변

    1. The cell pellet can be stored at -80°C with a small amount of PBS, avoiding storage with culture medium. Upon thawing, it's recommended to wash the cells once with PBS and then remove the PBS. Following this step, the assay buffer should be used for extraction according to the kit instructions. It's important to avoid multiple freeze-thaw cycles. However, the supplier does not specify the maximum time frozen cells will remain stable for assay.

      도움이 되었습니까?

후기

평점 값 없음

활성 필터

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.