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CAT100

Sigma-Aldrich

Catalase Assay Kit

sufficient for ≥100 tests enzymatic, determination of catalase activity in tissues and cells

동의어(들):

Catalase Activity Detection Kit

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1 KIT
₩2,015,181

₩2,015,181

출고 가능일2025년 4월 09일세부사항

벌크 견적 요청
모든 사진(1)

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1 KIT
₩2,015,181

About This Item

UNSPSC 코드:
12161503
NACRES:
NA.84

₩2,015,181

출고 가능일2025년 4월 09일세부사항

벌크 견적 요청

Quality Level

200

사용

sufficient for ≥100 tests enzymatic

검출 방법

colorimetric

배송 상태

wet ice

저장 온도

2-8°C

유전자 정보

human ... CAT(847)

관련 카테고리

일반 설명

The Catalase Assay Kit contains all necessary components for studying catalase activity in various tissues and subcellular organelles.

애플리케이션

Sutitable for Colorimetric and UV Assays

생화학적/생리학적 작용

Catalase is a ubiquitous antioxidant enzyme which catalyses the decomposition of hydrogen peroxide (H2O2) to water and oxygen. Hydrogen peroxide is formed in the eukaryotic cell as a by-product of various oxidases and superoxide dismutases. Hydrogen peroxide accumulation in cells causes oxidation of cellular targets such as DNA, proteins, and lipids leading to mutagenesis and cell death. Removal of the H2O2 from the cell by catalase provides protection against oxidative damage to living cells and its role in oxidative stress related diseases has been widely studied.

특징 및 장점

  • Useful for determining catalase activity - may be used in various tissues and cells
  • Simple, optimized protocol - A simple colorimetric assay for analysis of peroxisome enrichment and catalase activity

적합성

Suitable for studying catalase activity in various tissues and subcellular organelles.

원리

This assay method is based on the measurement of the hydrogen peroxide substrate remaining after the action of catalase. First, the catalase converts hydrogen peroxide to water and oxygen (catalatic pathway) and then this enzymatic reaction is stopped with sodium azide. An aliquot of the reaction mix is then assayed for the amount of hydrogen peroxide remaining by a colorimetric method.10 The colorimetric method uses a substituted phenol (3,5-dichloro-2-hydroxybenzenesulfonic acid), which couples oxidatively to 4-aminoantipyrine in the presence of hydrogen peroxide and horseradish peroxidase (HRP) to give a red quinoneimine dye (N-(4-antipyryl)-3-chloro-5-sulfonatep-benzoquinone-monoimine) that absorbs at 520 nm

단위 정의

One unit of catalase will decompose 1.0 micromole of hydrogen peroxide to oxygen and water per minute at pH 7.0 at 25 °C at a substrate concentration of 50 mM hydrogen peroxide.

제조 메모

Use ultrapure water in preparation of all solutions.

키트 구성품 역시 별도로 이용 가능함

제품 번호
설명
SDS
  • P6782Peroxidase from horseradish, Type VI-A, essentially salt-free, lyophilized powder, ≥250 units/mg solid (using pyrogallol), 950-2000 units/mg solid (using ABTS) 5 mgSDS
  • 323381Hydrogen peroxide solution, contains ~200 ppm acetanilide as stabilizer, 3 wt. % in H2OSDS

픽토그램

Health hazard
GHS08

신호어

Danger

유해 및 위험 성명서

H334

예방조치 성명서

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
0000389380
0000389380
0000198880
0000198880
0000160559

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COA 검색

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문서 라이브러리 방문

이미 열람한 고객

Slide 1 of 1

1 of 1

Peroxide Assay Kit sufficient for 250 colorimetric tests

Sigma-Aldrich

MAK311

Peroxide Assay Kit

A J Kowaltowski et al.
FEBS letters, 473(2), 177-182 (2000-05-17)
The involvement of reactive oxygen species in Ca(2+)-induced mitochondrial membrane permeabilization and cell viability was studied using yeast cells in which the thioredoxin peroxidase (TPx) gene was disrupted and/or catalase was inhibited by 3-amino-1,2, 4-triazole (ATZ) treatment. Wild-type Saccharomyces cerevisiae
Susmita Das Nishu et al.
PloS one, 14(3), e0213370-e0213370 (2019-03-13)
Algicidal bacteria have received broad acceptance as an ecofriendly tool for controlling harmful algal blooms. However, their practical application is still limited to the lab-scale tests due to the complex alga-bacterium interactions in different nutrient statuses. In this study, the
M E Tome et al.
Cancer research, 61(6), 2766-2773 (2001-04-06)
Glucocorticoids are used for the treatment of lymphoid neoplasms, taking advantage of the well-known ability of these compounds to cause apoptosis in lymphoid tissues. Previously, we have shown that dexamethasone, a synthetic glucocorticoid, causes a down-regulation of several antioxidant defense
S Tada-Oikawa et al.
FEBS letters, 442(1), 65-69 (1999-01-29)
Pulsed field gel electrophoresis showed that the initiation time of DNA breakage induced by the DNA alkylating agent duocarmycin A, which is not a redox-cycling agent, was almost the same in the human leukemia cell line HL-60 and its H2O2-resistant
M Zámocký et al.
Progress in biophysics and molecular biology, 72(1), 19-66 (1999-08-14)
This review gives an overview about the structural organisation of different evolutionary lines of all enzymes capable of efficient dismutation of hydrogen peroxide. Major potential applications in biotechnology and clinical medicine justify further investigations. According to structural and functional similarities
모든 관련된 서류 보기

문서

Organelle Isolation

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Mitochondrial Stress and ROS

Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.

질문

1–2 / 2 질문  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 답변

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 답변

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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