10110434001

Xanthine Oxidase (XOD)

from cow milk

• 동의어(들): XOD
• 효소 위원회 번호: 1.1.3.22 (BRENDA, IUBMB)
• UNSPSC 코드: 12352204

속성

• 생물학적 소스: bovine milk
• Quality Level: 100
• 양식: suspension
• 특이 활성도: ~1 units/mg protein (At 25 °C with xanthine as the substrate.)
• 포장: pkg of 1 mL (20 U)
• 제조업체/상표: Roche
• 최적 pH: 8.5-9.0
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

일반 설명

Xanthine Oxidase (XOD) is a metal flavoprotein. It has FAD, molybdenum and iron in the ratio 2:2:8.

Xanthine:oxygen oxidoreductase

애플리케이션

Xanthine Oxidase (XOD) has been used in the assessment of XOR-mediated NO production from NDHP and assessment of nitrite-derived NO in liver and purified XOR.

Xanthine Oxidase has been used to study tyrosine nitration.

생화학적/생리학적 작용

Xanthine Oxidase (XOD) exhibits a broad substrate specificity including aldehydes, purines and pteridines. Furthermore, this enzyme reduces oxygen to generate superoxide, hydrogen peroxide and reactive oxygen species (ROS). It also reduces nitrite to yield reactive nitrogen species (RNS), such as peroxynitrite and nitric oxide. Owing to its ability to generate RNS and ROS, XOD might play an important role as an antimicrobial agent in the neonatal gut, thereby complementing endogenous enzyme of the intestinal epithelium.

품질

Contaminants: <0.005% guanase, NP and uricase, each, <0.05% ADA, <0.05% alkaline phosphatase (4-nitrophenyl phosphate as the substrate)
Note: Chromatographically purified.

서열

XOD is a dimer. Each subunit contains 1 atom of molybdenum, 2 iron-sulfur centers (non-heme iron, ferredoxin-type) and 1 molecule of FAD.

단위 정의

One unit (U) xanthine oxidase will produce 1 μmol of uric acid (E_293nm = 12.2 mmol ^-1 x L x cm^-1) from the oxidation of 1 μmol of xanthine in 1 min at 25 °C and pH8.5.

물리적 형태

Suspension in 3.2 M ammonium sulfate solution, 10 mM EDTA, pH approximately 8

제조 메모

Activator: O₂
Stabilizers: The substance is stabilized by the addition of EDTA; salicylate is not added.

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash