X4500

Xanthine Oxidase from bovine milk

Grade III, ammonium sulfate suspension, ≥0.8 units/mg protein

• 동의어(들): Schardinger enzyme, XnOx, hypoxanthine oxidase, XOD, Xanthine:oxygen oxidoreductase
• CAS Number: 9002-17-9
• 효소 위원회 번호: 1.17.3.2.
• EC Number: 232-657-6
• MDL number: MFCD00082145
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: bovine milk
• 유형: Grade III
• 형태: ammonium sulfate suspension
• 특이 활성도: ≥0.8 units/mg protein
• 기술: inhibition assay: suitable
• 색상: faint brown to dark brown
• UniProt 수납 번호: A0A3Q1LW91; A0A3Q1MM92; A0A3S5ZPW7
• 외래 활성: uricase ≤0.1%
• 저장 온도: 2-8°C

설명

일반 설명

Research area: Cell Cycle

Xanthine Oxidase (XO) belongs to the class of complex metalloflavoproteins. It is produced by oxidation of sulfhydryl residues or by proteolysis of xanthine dehydrogenase (XDH). XO is characterized with two flavin molecules (FAD), two molybdenum atoms, and eight iron atoms bound per enzymatic unit.

Formerly E.C. 1.1.3.22

애플리케이션

Xanthine Oxidase from bovine milk has been used:

• as a source for superoxide generation
• for the degradation of hypoxanthine
• for the detection of reactive oxygen species (ROS) in lipid model by electron spin resonance (ESR) spin trap method
• as a constituent of assay mixture in xanthine oxidase inhibition assay

생화학적/생리학적 작용

Xanthine Oxidase (XO)catalyzes the hydroxylation of hypoxanthine to xanthine and xanthine to uric acid. Xanthine oxidase activity is inhibited by folic acid. [7] and various other inhibitors including 3,4-Dihydroxy-5-nitrobenzaldehyde (DHNB), febuxostat and allopurinol. XO and xanthine dehydrogenase (XDH) plays a vital role in the last two steps in the formation of urate. Elevated levels of XO has been observed in the serum of chronic liver disease patients. Therefore, XO can be used as a biomarker for the detection of liver disease.

Xanthine oxidase activity is inhibited by folic acid.

Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species.

단위 정의

One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.

물리적 형태

Suspension in 2.3 M (NH₄)₂SO₄, 10 mM sodium phosphate buffer, pH 7.8, containing 1 mM EDTA and 1 mM sodium salicylate

제조 메모

Chromatographically purified

저장 및 안정성

Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons

분석 메모

Protein determined by biuret

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P304 + P340 + P312 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)