The product is a native protien from Arthrobacter globiformis. This material has not been sequenced. However, this information is published at UniProt.
Please see the link below to review:
https://www.uniprot.org/uniprot/D0VWQ1
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フォーム
lyophilized powder
比活性
15-30 units/mg protein (biuret)
分子量
tetramer ~32 kDa
保管温度
−20°C
SMILES記法
[P](=O)([O-])(OC3C(OC(C3O)N4C=CC(=O)NC4=O)CO)OCC1OC(C(C1O)O)N2C=CC(=O)NC2=O.[N+H4]
InChI
1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3
InChI Key
VPXDHZMLJOJKOX-UHFFFAOYSA-N
詳細
Research area: Cell Signalling. Uricase is made of four identical subunits and is a homotetrameric enzyme. It is found as a functional uricase in several organisms including, bacteria, archaea, and eukaryotes. Nonetheless, primates and humans lack a functional uricase enzyme.[1]
アプリケーション
生物化学的／生理学的作用
Arthrobacter globiformis is a major producer of uricase or urate oxidase in poultry manure and litter. Uricase hydrolyzes uric acid and causes ammonia volatilization in poultry manure.[3] In the majority of mammals, uricase converts uric acid to allantoin, a soluble excretion product. Humans lack uricase due to its loss during the evolution of hominids.[4] This increases the risk of humans towards gout, kidney stones, hypertension, and diabetes due to increased circulating levels of uric acid.[5]Uricase is an important enzyme that plays a role in the purine metabolic pathway.[6]
Molecular weight: 4 × ~32 KDa (tetramer)
Isoelectric point: is 4.64
KM value (uric acid as substrate): ~ 0.01 mM
The optimum pH for activity: 8.5-9.5
The optimum pH for stability: 6.0-9.5
Isoelectric point: is 4.64
KM value (uric acid as substrate): ~ 0.01 mM
The optimum pH for activity: 8.5-9.5
The optimum pH for stability: 6.0-9.5
Zn and Cu were shown to inhibit the activity of uricase. [7]
単位の定義
1ユニットは、pH 9.0、25°C、1分間で、1.0 μmolの尿酸をアラントインに変換する酵素量です。
シグナルワード
Danger
危険有害性情報
危険有害性の分類
Resp. Sens. 1
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
U7128-100UN:
U7128-BULK:
U7128-VAR:
U7128-10UN:
U7128-25UN:
この製品を見ている人はこちらもチェック
A Therapeutic Uricase with Reduced Immunogenicity Risk and Improved Development Properties
Andrew C N, et al.
PLoS ONE, 11(12) (2016)
Development of a sandwich format, amperometric screen-printed uric acid biosensor for urine analysis.
Kanyong P, et al.
Analytical biochemistry, 428(1), 39-43 (2012)
Review of Uricase and Other Key Metabolites Produced by Microorganisms through Submerged Fermentation INTRODUCTION
Sandeep V C, et al.
Fluoropolymers 2. , 22(4) (2023)
Uric acid: a danger signal from the RNA world that may have a role in the epidemic of obesity, metabolic syndrome, and cardiorenal disease: evolutionary considerations.
Seminars in Nephrology, 31(5), 394-399 (2011)
Effect of minerals on activity of microbial uricase to reduce ammonia volatilization in poultry manure.
Kim WK and Patterson PH
Poultry Science, 82(2), 223-231 (2003)
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Hello, I would like to get theprotein sequence of the Arthrobacter globiformis urucase, catalog U7128. Is it a recombinant enzmye made in E. coli, or is it a native prurified protein from the A. globiformis host. Thank you. Guillaume
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アクティブなフィルタ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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