slides can be dried by leaving them at room temperature overnight or by heating them at 37-40 degrees C for several hours. However, it is standard lab policy to wait at least 3 or 4 days before filing the slides, especially if excess mounting media was used. Storing slides in a cabinet before they are fully dry could lead to them sticking together.
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環境により配慮した代替製品の特徴
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
色
clear
密度
0.840 g/cm3
アプリケーション
hematology
histology
環境により配慮した代替製品カテゴリ
保管温度
room temp
アプリケーション
Organo/Limonene Mount™ is a ready-to-use mounting medium is made with limonene, a natural product from orange peels. It is good for coverslipping stained tissues and cell smears that can be dehydrated with organic solvents.[1][2][3] This mounting medium is suitable for organic solvent resistant immunohistochemistry (IHC) chromogens as well as an excellent choice for mounting H and E stained slides.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to toluene based mounting mediums.
関連事項
Organo/Limonene Mount™ is comparable to Clarion™ mounting medium.
法的情報
Clarion is a trademark of Biomeda Corp.
Organo/Limonene Mount is a trademark of ImmunoBioScience Corp.
シグナルワード
Danger
危険有害性情報
危険有害性の分類
Aquatic Acute 1 - Aquatic Chronic 1 - Asp. Tox. 1 - Flam. Liq. 3 - Skin Irrit. 2 - Skin Sens. 1
保管分類コード
3 - Flammable liquids
WGK
WGK 3
引火点(°F)
118.4 °F
引火点(℃)
48 °C
個人用保護具 (PPE)
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
消防法
第4類:引火性液体
第二石油類
危険等級III
非水溶性液体
労働安全衛生法名称等を表示すべき危険物及び有害物
名称等を表示すべき危険物及び有害物
労働安全衛生法名称等を通知すべき危険物及び有害物
名称等を通知すべき危険物及び有害物
Jan Code
O8015-VAR:
O8015-30ML-PW:
O8015-BULK:
O8015-30ML:
O8015-100ML-PW:
O8015-100ML:
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Impaired Interoception in a Preclinical Model of Functional Dyspepsia.
Winston J H, et al.
Digestive Diseases and Sciences, 62(9), 2327-2337 (2017)
Supplementation of specific carbohydrates results in enhanced deposition of chondrogenic-specific matrix during mesenchymal stem cell differentiation.
Trudy C W, et al.
Journal of Tissue Engineering and Regenerative Medicine, 15(5), 1261-1272 (2018)
HDAC11 Suppresses the Thermogenic Program of Adipose Tissue via BRD2.
Bagchi R A, et al.
bioRxiv, 292011-292011 (2018)
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Transdermal drug delivery uses chemical, physical, or biochemical enhancers to cross the skin barrier. However, existing platforms require high doses of chemical enhancers or sophisticated equipment, use fragile biomolecules, or are limited to a certain type of drug. Here, we
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How long does it take for slides to dry when coverslipped with Organo/Limonene O8015?
1 回答-
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Is the organo/limonene mounting media a hard-setting media, or does it require extra sealing on the coverslip (i.e. nail polish)?
1 回答-
This product is a hard-setting mounting medium, sealing is not required. Simply add 2-3 drops of mounting medium and carefully apply the coverslip, avoiding air bubbles. Slides may be dried overnight at room temperature or by incubating at 37-40°C for several hours.
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What are the instructions on preparing a sample for use with Product No. O8015 (Organo/Limonene Mount™)?
1 回答-
Dehydrate slides with dehydrating agents, such as graded ethanol or reagent alcohol. Dehydration refers to removing the water by using a series of grades alcohols. Typically slides spend 1-5 minutes each in a series of alcohols. Sequences vary by laboratory. A typical sequence would be 1 station of 80% alcohol, 2-3 changes of 95% alcohol, 2-3 changes of absolute alcohol. The slides are then processed through 2-3 changes of xylene. After finishing with the last xylene solution, the slides are ready for coverslipping with the Organo/Limonene Mount.
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Organo/Limonene Mount (Product No. O8015) is stated to be suitable for use with chromogens for immunohistochemistry. Does this?
1 回答-
Suitable chromogens for immunohistochemistry would be DAB, or DAB with an added metal such as nickel or cobalt. Alkaline phosphatase chromogens would also be suitable for use with Oregano/Limonene Mount.
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How does the storage temperature relate to shipping conditions?
1 回答-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
役に立ちましたか？
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The instructions for use of Product No. O8015, Organo/Limonene Mount™, call for using xylene for clearing. Can limonene or other xylene substitutes be considered acceptable for use?
1 回答-
Yes, limonene would be considered a suitable xylene substitute. There are however a wide variety of xylene substitutes available on the market. No exhaustive comparative studies have been performed using Organo/Limonene Mount with each and every available xylene substitute.
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Why is Organo/Limonene Mount (Product No. O8015) preferable to a mounting media that is xylene based?
1 回答-
There are more health hazards associated with xylene in comparison to products derived from natural products, such as Organo/Limonene Mount.
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