The protocol for cell samples using MAK434 is as follows:
- Suspend about two million (2 × 10^6) harvested cells in 400 μL PBS on ice. Lysis can be achieved by homogenization (10-20 passes in a Dounce homogenizer on ice) or by sonication (preferably performed in an ice-water bath). The degree of cell lysis can be checked under a microscope. Centrifuge the homogenate at 14,000 g for 10 min and transfer the clear supernatant into a clean tube. It is advisable to run a pilot test of the sample at different dilutions and select a dilution with readings in the linear range of the standard curve for further assays. Most samples can be stored at -80°C if not assayed immediately.
- If a lysis buffer is to be used, it is recommended to first run an assay to test if components in the lysis buffer interfere with the assay. This can be accomplished by running two standard curves: one prepared in H2O and one prepared in the same proportion of lysis buffer that the sample will contain. If the two standard curves are the same, there is no interference and it is safe to use the lysis buffer. If there is a difference in the standard curves, the lysis buffer may still be used if the standard curve remains linear; however, the standard curve to be used for analyzing the lysate samples will need to be prepared in the lysis buffer.
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品質水準
入力
cell culture medium
tissue
serum
urine
food(s)
beverage(s)
plasma
アプリケーション
agriculture
cosmetics
environmental
food and beverages
検出方法
colorimetric
関連疾患
gastrointestinal diseases
保管温度
−20°C
詳細
Acetaldehyde (CH₃CHO) is a commonly found aldehyde in nature and widely used in various industries. It is a metabolic byproduct of ethanol in the liver and is toxic to the human body. However, it is rapidly converted to the less harmful acetic acid by the enzyme aldehyde dehydrogenase. Individuals deficient in aldehyde dehydrogenase experience accumulation of acetaldehyde upon alcohol consumption, leading to facial and body flushing known as alcohol flush reaction or “Asian flush syndrome.” Acetaldehyde buildup has also been linked to alcohol-related hangover effects. Despite its classification as a carcinogen, acetaldehyde is naturally present in many foods and beverages such as ripe fruit, coffee, and wine.
アプリケーション
The Acetaldehyde Assay Kit may be used for:
- Alcohol Metabolism Research
- Hepatology Research
- Neurological Research
特徴および利点
- Simplified Process: Enjoy a hassle-free procedure that requires the addition of a single working reagent and only a 30 minute assay room temperature reaction, saving you time and effort.
- Convenient and Sensitive: Uses only 20 µL of sample for the determination of acetaldehyde with a detection range of 2 µM to 2mM.
- High-Throughput Compatibility: Seamlessly integrate our kit into high-throughput handling systems, ensuring efficiency and accuracy.
適合性
The kit is suitable for acetaldehyde determination in biological samples (e.g. plasma, serum, urine, tissue and culture media) and food/beverage samples (e.g. wine, coffee, and juice).
原理
The Acetaldehyde Assay Kit is based on the aldehyde dehydrogenase catalyzed oxidation of acetaldehyde with the reduction of NAD to NADH. The formed NADH subsequently reduces MTT, producing a colored formazan compound. The intensity of the end product color, measured at 565 nm, is directly proportional to the acetaldehyde concentration in the sample.
その他情報
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シグナルワード
Warning
危険有害性情報
危険有害性の分類
Eye Irrit. 2 - Skin Irrit. 2
保管分類コード
12 - Non Combustible Liquids
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
毒物及び劇物取締法
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PRTR
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消防法
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労働安全衛生法名称等を表示すべき危険物及び有害物
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カルタヘナ法
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Jan Code
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試験成績書（COA）
Lot/Batch Number
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How should MAK434 be used for cell samples?
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