Since certain components in 10X medium concentrates precipitate at pH 7.0, it is necessary to adjust the pH of these solutions to maintain solubility.
Therefore, it may be necessary for the user to adjust the pH of the 1X preparation with sterile 1 N NaOH or 1 N HCl. Procedure For Dilution to 1X (1 Liter):
(NOTE: Dilution of 10X solutions should be performed with sterile containers, components, and equipment.)
• Aseptically measure out approximately 850 ml of tissue culture grade water into an appropriate size container.
• While gently stirring the water, add 100 ml of 10X medium.
• To the solution, add the required amount of sodium bicarbonate ( 0.35 g/L) and L-glutamine (0.1 g/L), at 1X
• While stirring, adjust the solution to desired pH with 1 N NaOH or 1 N HCl.
• Bring medium to final volume with additional tissue culture grade water.
• It is recommended to sterile filter the prepared medium through a 0.22 ?m sterile membrane, or, for example, a Stericup.
• Aseptically dispense into sterile containers. Store refrigerated at 2-8° C
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製品名
イーグル最少必須培地, 10 ×, With Hanks′ salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
無菌性
sterile-filtered
フォーム
liquid
濃度
10 ×
テクニック
cell culture | mammalian: suitable
不純物
endotoxin, tested
構成
NaHCO3: no
Hanks’ salts (2% CO2): yes
L-glutamine: no
HEPES: no
sodium pyruvate: no
phenol red: yes
輸送温度
ambient
保管温度
2-8°C
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関連するカテゴリー
詳細
Minimum Essential Medium (MEM), one of the most used synthetic cell culture media, is developed by Harry Eagle. It is used to cultivate various types of cells grown in monolayers.
アプリケーション
Minimum Essential Medium Eagle has been used as a transport media to maintain pig liver cells.[1]
再構成
Supplement with 0.292 g/L L-glutamine, 0.35 g/L sodium bicarbonate at 1×.
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製品番号
詳細
価格
栄養補助剤
製品番号
詳細
価格
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
M9288-500ML:
M9288-BULK:
M9288-100ML:
M9288-VAR:
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資料
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
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If I have 10x Medium, how can I dilute it in order to have 1x and grow cells?
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