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M5519

Sigma-Aldrich

ムラシゲ・スクーグ基礎培地

powder, suitable for plant cell culture

別名:

MS基礎培地

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サイズを選択してください

1 L
￥2,090
10 L
￥5,860
50 L
￥20,200

￥2,090

出荷可能日2025年4月09日詳細

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1 L
￥2,090
10 L
￥5,860
50 L
￥20,200

About This Item

MDL番号:
MFCD00240976
UNSPSCコード:
12352207
NACRES:
NA.72

￥2,090

出荷可能日2025年4月09日詳細

バルクの問い合わせ

フォーム

powder

テクニック

cell culture | plant: suitable

アプリケーション

agriculture

保管温度

2-8°C

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関連するカテゴリー

詳細

Murashige and Skoog培地は、広く使用されている植物組織培養増殖培地です。 M&S基礎培地には、高濃度の硝酸塩や寒天、糖、ビタミンおよび成長調整剤などの有機添加物を含む多量栄養素が含有されています。 M&Sに頻繁に添加される重要な成長調節剤には、IAA（オーキシン／モルフォゲン）およびカイネチン（サイトカイニン／細胞分裂促進剤）などがあります。

アプリケーション

MurashigeおよびSkoog培地は、植物の形質転換研究用に植物組織培養培地に使用されています。[1][2]

その他の調製法

多量栄養素と微量栄養素、およびMurashigeとSkoogが報告（1962年）したビタミン含有。

培地組成

数量

4.4 gの粉末で1 Lの培地ができる配合です。

調製ノート

ムラシゲ・スクーグ培地は粉末から再構成することも、完全ムラシゲ・スクーグ培地の主成分製品(多量養素混合液やビタミン混合液など)を混合して作成することもできます。ムラシゲ・スクーグ培地(M5519)には、オリジナルの古典的組成の多量養素とビタミンが含まれています。スクロース、寒天、オーキシン(IAA)およびサイトカイニン(キネチン)を加えて、植物組織培養の完全培地を調製することもできます。

関連製品

製品番号
詳細
価格

08168

Timestrip Plus™ 8°C

ピクトグラム

Flame over circleExclamation mark
GHS03,GHS07

シグナルワード

Warning

危険有害性情報

H272,H319

危険有害性の分類

Eye Irrit. 2 - Ox. Sol. 3

保管分類コード

5.1B - Oxidizing hazardous materials

WGK

WGK 2

引火点(°F)

Not applicable

引火点(℃)

Not applicable

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

PRTR

第一種指定化学物質

労働安全衛生法名称等を表示すべき危険物及び有害物

名称等を表示すべき危険物及び有害物

労働安全衛生法名称等を通知すべき危険物及び有害物

名称等を通知すべき危険物及び有害物

Jan Code

M5519-50L:4548173305073
M5519-1L-LBL:
M5519-10L-LBL:
M5519-25KG:
M5519-50K3382:
M5519-50.1K:
M5519-25K:
M5519-BIBC:
M5519-10L:4548173305080
M5519-BG:
M5519-10L-PW:
M5519-50L-LBL:
M5519-1L:4548173305066
M5519-50K:
M5519-BULK:
M5519-PROC:
M5519-50KG:
M5519-50L-PW:
M5519-1L-PW:
M5519-VAR:
M5519-1L-KC:

最新バージョンのいずれかを選択してください：

試験成績書（COA）

Lot/Batch Number
0000422941
0000422941
0000366116
0000366116
0000390198

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特定のバージョンが必要な場合は、ロット番号またはバッチ番号で特定の証明書を検索できます。

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以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

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Youjun Zhang et al.
Plant communications, 1(5), 100028-100028 (2020-12-29)
Although the use of stable transformation technology has led to great insight into gene function, its application in high-throughput studies remains arduous. Agro-infiltration have been widely used in species such as Nicotiana benthamiana for the rapid detection of gene expression and
Thierry G A Lonhienne et al.
Scientific reports, 4, 5055-5055 (2014-05-24)
Growth, morphogenesis and function of roots are influenced by the concentration and form of nutrients present in soils, including low molecular mass inorganic N (IN, ammonium, nitrate) and organic N (ON, e.g. amino acids). Proteins, ON of high molecular mass
Liang Zhu Y et al.
Plant physiology, 119(1), 73-80 (1999-01-08)
An important pathway by which plants detoxify heavy metals is through sequestration with heavy-metal-binding peptides called phytochelatins or their precursor, glutathione. To identify limiting factors for heavy-metal accumulation and tolerance, and to develop transgenic plants with an increased capacity to
Jingyi Wang et al.
Scientific reports, 9(1), 141-141 (2019-01-16)
Abiotic stress significantly impacts growth and yield of crop plants. It is imperative for crop improvement to discover and utilize stress-tolerant functional genes. In this study, genes responding to abiotic stresses, such as freezing, salt and osmotic stress, were screened
Sina Fischer et al.
Journal of experimental botany, 72(2), 415-425 (2020-10-11)
High Arsenic Concentration 1 (HAC1), an Arabidopsis thaliana arsenate reductase, plays a key role in arsenate [As(V)] tolerance. Through conversion of As(V) to arsenite [As(III)], HAC1 enables As(III) export from roots, and restricts translocation of As(V) to shoots. To probe
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質問

1–10/11 質問  

  1. How do I prepare this medium?

    1 回答

    1. The preparation instructions for this product are as follows:
      1. Using a container twice the size of the desired final volume, measure out ~90% of the required final volume of tissue culture grade water (e.g., Catalog Number W3500). Example: 900 ml for a final volume of 1000 ml.
      2. While stirring, add the powdered product.
      3. Rinse the original container with a small volume of tissue culture grade water to remove traces of the powder. Add to the solution in step 2.
      4. Add desired supplements (e.g., sucrose, gelling agent, auxins, cytokinins).
      Add heat labile constituents after autoclaving.
      5. While stirring, adjust to the desired pH (e.g., 5.7±0.1) using KOH, NaOH, or HCl.
      6. Add additional tissue culture grade water to bring the medium to the final volume.
      7. If a gelling agent is used, heat the solution to clarity while stirring.
      8. Dispense the medium into culture vessels before or after autoclaving according to the application.
      Add heat labile constituents after autoclaving.
      9. Sterilize the medium in a validated autoclave at 1 kg/cm2 (15 psi). The medium should attain a temperature of 121 °C for at least 15 minutes.

      Please see the link below to review this and additional information available in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/361/537/m5519pis.pdf

      役に立ちましたか？

  2. What is the difference between Product M5519, Murashige and Skoog (MS) Basal Medium and M5524, Murashige and Skoog Basal Salt Mixture?

    1 回答

    1. Product M5524 is the Murashige and Skoog(M&S) Basal Salt Mixture, only. The M5519 products is M&S Basal Medium which also includes the vitamins myo-Inositol, Nicotinic acid and Pyridoxine HCl. These formulations are identical with the exception of vitamin components. With the addition of 1 mL of M7150 M&S Vitamin Solution, the composition of each medium is equivalent.

      役に立ちましたか？

  3. What is the formulation of Product M5519, Murashige and Skoog (MS) Basal Medium?

    1 回答

    1. Please see the link below to review the product datasheet, including the full formulation:

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/361/537/m5519pis.pdf

      To review additional Murashige and Skoog formulations, please see the link below:

      https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/plant-tissue-culture/murashige-skoog

      役に立ちましたか？

  4. Product M5519, Murashige and Skoog Basal Medium, is on back order. Can you suggest an alternate?

    1 回答

    1. Product M5524 Murashige and Skoog(M&S) Basal Salt Mixture, is an ideal alternative to M5519, M&S Basal Medium. These formulations are identical with the exception of vitamin components. With the addition of 1 mL of M7150 M&S Vitamin Solution, the composition of each medium is equivalent.

      役に立ちましたか？

  5. Can I autoclave Product M5519, Murashige and Skoog Basal Medium?

    1 回答

    1. Yes, this medium is autoclavable.

      役に立ちましたか？

  6. once the media is prepared, what conditions should it be stored in, and how long can it be stored?

    1 回答

    1. The prepared solution is quite stable but should be stored refrigerated. Monitor the salt solution for particulates or turbidity. After the addition of agar and subsequent sterilization, poured plates or vessels should also be stored aseptically in the refrigerator until use. The shelf life of the stored plates or vessels is most often limited by less stable compounds added to the medium. The end user would have to determine the stability of these components. While there is no firm shelf life on the prepared medium, contamination, and drying are the most common reasons for degradation. Monitor plates/vessels for contaminants or turbidity. An indication of excessive drying will be the gelled medium pulling away from the sides of the plate or vessel. The medium has lost moisture and may not be suitable for use. Moisture loss can be minimized by storing prepared plates/vessels in a sealed container or sealed bags. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/361/537/m5519pis.pdf

      役に立ちましたか？

  7. What is the percentage of sucrose in M5519?

    1 回答

    1. The M5519 does not contain sucrose. This component would have to be added prior to autoclaving. The typical amount is 30 g/L. See the link below to review the product information sheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/361/537/m5519pis.pdf

      Should sucrose be desired, see product M9274, which is more of a complete formulation containing M&S salts, vitamins, sucrose, and agar. See the link below to review the formulations of the various Murashige & Skoog media:
      https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/plant-tissue-culture/murashige-skoog#Basal%20Salts%20Mixtures

      役に立ちましたか？

  8. What is the quantity and what is shelf life

    1 回答

    1. The SKU sizes indicated refer to the final volume of media that can be made upon reconstitution of the pre-weighted powder. This powdered media is formulated to contain 4.4 grams of powder per liter of medium. This product does not contain an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services

      役に立ちましたか？

  9. How much agar is there in Product M5519, Murashige and Skoog Basal Medium?

    1 回答

    1. This product does not contain any agar.

      役に立ちましたか？

  10. What is the Department of Transportation shipping information for this product?

    1 回答

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      役に立ちましたか？

1–10/11 質問  

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