Yes, gelation is pH dependent. Adjusting the pH to 5.7 is recommended prior to heating to melt the agar and autoclaving to sterilize. Please see the link below to review the general plant culture medium preparation instructions available in the datasheet for product M5519 (formulation without agar):
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/361/537/m5519pis.pdf
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シグナルワード
Warning
危険有害性情報
危険有害性の分類
Eye Irrit. 2 - Ox. Sol. 3
保管分類コード
5.1B - Oxidizing hazardous materials
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
PRTR
第一種指定化学物質
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Jan Code
M9274-BULK:
M9274-PROC:
M9274-1L-LBL:
M9274-1L-C:
M9274-1L-PW:
M9274-1L:
M9274-10L-PW:
M9274-2X5L:
M9274-10L:
M9274-VAR:
M9274-10L-LBL:
最新バージョンのいずれかを選択してください：
試験成績書（COA）
Lot/Batch Number
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Antoine Fort et al.
The New phytologist, 209(2), 590-599 (2015-09-24)
Heterosis is the phenomenon whereby hybrid offspring of genetically divergent parents display superior characteristics compared with their parents. Although hybridity and polyploidy can influence heterosis in hybrid plants, the differential contributions of hybridity vs polyploidy to heterosis effects remain unknown.
Striking effects of melatonin on secondary metabolites produced by callus culture of rosemary (Rosmarinus officinalis L.)
Coskun Y, et al.
Plant Cell, Tissue and Organ Culture, 138(1), 89-95 (2019)
Baba Ngom et al.
Plant signaling & behavior, 12(9), e1356967-e1356967 (2017-08-02)
Cytosine DNA methylation is an epigenetic regulatory system used by plants to control gene expression. Methylation pattern always changes after abiotic stresses, pathogens and pest infections or after a treatment with salicylic acid (SA). The latter is a key player
Laure Audonnet et al.
Gene expression patterns : GEP, 25-26, 1-7 (2017-04-13)
Dimethylation of histone H3 lysine 9 (H3K9me2) is a heterochromatic mark linked to DNA methylation and gene repression. Removal of H3K9me2 from gene bodies by the jmjC histone demethylase IBM1/JMJ25 inhibits DNA methylation and derepresses gene expression. In this work
K Sopalun et al.
Cryo letters, 31(4), 347-357 (2010-09-08)
Three vitrification-based methods for the cryopreservation of Grammatophyllum speciosum protocorms were invesigated: droplet-vitrification, encapsulation-dehydration and encapsulation-vitrification. Protocorms, 0.1 cm in diameter, developed from 2-month-old germinating seeds were used. For droplet-vitrification, protocorms were precultured on filter paper soaked in half strength
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Would an incorrect pH level affect the setting of the agar?
1 回答-
役に立ちましたか？
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How firm is the gel in the final sterile medium preparation of Murashige and Skoog Basal Medium, Product M9274?
1 回答-
This formulation contains 8g/L of standard agar resulting in fairly firm and rigid culture medium.
役に立ちましたか？
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
役に立ちましたか？
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What is the stability of the prepared Murashige and Skoog Basal Medium, Product M9274?
1 回答-
This agar-containing medium can be stored for 3 to 6 months, depending on the stability of any additional supplements, if stored properly with no observable dehydration of the gel. If the medium appears to darken or pull away from the sides of the culture vessel, it should be discarded.
役に立ちましたか？
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How is Murashige and Skoog Basal Medium, Product M9274 different from Product M5519?
1 回答-
M9274 contains sucrose at 30 g/L and agar at 8 g/L; M5519 does not.
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