おすすめの製品
ligand
Cibacron Blue
包装
pack of 50 mL
メーカー/製品名
Cytiva 17-0948-01
保管条件
(20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8.0)
Matrix
6% cross-linked agarose
粒径
45-165 μm
平均直径
90 μm
cleaning in place
3-13
動作範囲
4-12
適合性
suitable for bioprocess medium
保管温度
2-8°C
詳細
Blue Sepharose™ 6 Fast Flow is a member of the Cytiva range of affinity chromatography media for capture and intermediate purification.
Blue Sepharose™ 6 Fast Flow is composed of cross-linked 6% agarose beads modified with Cibacron Blue 3G covalently attached by the triazine coupling method.
The blue dye binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+.
Blue Sepharose™ 6 Fast Flow has been developed and tested in co-operation with leading large scale manufacturers and is used in routine commercial production.
Blue Sepharose™ 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, Blue Sepharose™ 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
特徴および利点
- Designed for large-scale purification of interferon and albumin. Also used for isolating groups of enzymes and removing of albumin
- BioProcess medium supported for industrial applications and well-established in approved processes
- Cibacron Blue 3G covalently coupled to the base matrix allowing for low ligand leakage and high capacity
保管および安定性
アナリシスノート
法的情報
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
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資料
This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
プロトコル
Remove albumin from affinity chromatography samples using HiTrap™ Blue HP or Blue Sepharose® 6 Fast Flow from Cytiva.
This page shows how to separate or remove albumin by affinity chromatography using HiTrap Blue HP and Blue Sepharose 6 Fast Flow.
Affinity chromatography purification of enzymes using 5’ AMP Sepharose® 4B, HiTrap® Blue HP, and Blue Sepharose® 6 Fast Flow products.
This page shows how purify or remove albumin with Blue Sepharose and Capto Blue from Cytiva.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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