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安全性情報

C-44010

Sigma-Aldrich

Lymphocyte Separation Medium 1077

500 ml

別名:

Lymphocyte Medium, Separation Medium for Lymphocytes

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About This Item

UNSPSCコード:
12352207
NACRES:
NA.71
現在、価格および在庫状況を閲覧できません。

フォーム

aqueous solution

包装

pkg of 500 ml

テクニック

cell culture | mammalian: suitable

輸送温度

ambient

保管温度

room temp

詳細

Lymphocyte Separation Medium 1077

アプリケーション

Lymphocyte Separation Medium 1077 is intended for the separation of the portion of vital mononuclear cells from whole blood, buffy coats, bone marrow and several other starting materials, e.g. crude cell preparations, by means of low density gradient centrifugation. Lymphocyte separation Medium has a density of 1.077 g/ml at 20°C. It is sterile, ready-to use and based on Ficoll 400 and sodium diatrizoate providing optimal physiological parameters and low cytotoxicity.

品質

All lots of PromoCell Lymphocyte Separation Medium 1077 are subjected to comprehensive quality control tests. Each lot is routinely tested for function and physical parameters such as osmolality and pH level. Approved in-house lots are used as a reference. In addition, all lots have been tested for the absence of microbial contaminants (fungi, bacteria).

保管および安定性

Lymphocyte Separation Medium 1077 is light-sensitive! Store the medium at room temperature in the dark immediately after arrival. Do not freeze. If stored properly, the product is stable until the expiry date stated on the label.

継代と培養方法

Click here for more information.

ピクトグラム

Corrosion
GHS05

シグナルワード

Warning

危険有害性情報

H290,H315,H319

危険有害性の分類

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

保管分類コード

8B - Non-combustible corrosive hazardous materials

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

労働安全衛生法名称等を表示すべき危険物及び有害物

名称等を表示すべき危険物及び有害物

労働安全衛生法名称等を通知すべき危険物及び有害物

名称等を通知すべき危険物及び有害物

Jan Code

C-44010:4548173376103

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試験成績書（COA）

Lot/Batch Number

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ウシ胎児血清 non-USA origin, from USDA approved countries, Heat Inactivated, sterile-filtered, suitable for cell culture

Sigma-Aldrich

12306C

ウシ胎児血清

Histopaque®-1119 sterile-filtered, density: 1.119

Sigma-Aldrich

11191

Histopaque®-1119

Fengyu Ming et al.
BioMed research international, 2020, 4658109-4658109 (2020-10-09)
Parkinson's disease (PD) is the second most common neurodegenerative disease in middle-aged and elderly people. However, the etiology and pathogenesis of PD are still unclear and there is a lack of reliable biomarkers for early molecular diagnosis. Parkin (encoded by

資料

Primary Human Mononuclear Cells Culture

Umbilical cord blood and adult peripheral blood mononuclear cells with optimized maintenance medium for short-term cultivation.

質問

1–2/2 質問  

  1. What's the procedure of lymphocytes separation with this media and the quantity used .

    1 回答

    1. This is a product of PromoCell. Please see the instructions for use provided by the manufacturer:
      1. Fill Lymphocyte Separation Medium 1077 in a tube. Use 3 ml for a 15 ml tube and 20 ml for a 50 ml tube.
      2. Hold the tube at a 45° angle and carefully load the cell suspension to be separated on top of the separation medium.
      Note: Perform this step slowly but constantly. Take care that the two phases do not mix and a sharp border is present.
      For best results, use an electrical pipet aid with EX-function allowing the slow and constant efflux of the cell suspension from the pipet.
      3. Centrifuge the tube at 440 x g for 40 minutes at room temperature without brake.
      4. Carefully aspirate most of the supernatant without disturbing the layer of mononuclear cells in the interphase.
      5. Aspirate the ring of mononuclear cells from the interphase using a pipet. Keep the volume as small as possible.
      6. Wash the mononuclear cells with PBS/0.1% HSA or BSA and centrifuge at 360 x g for 10 minutes.
      7. Wash the mononuclear cells with PBS/0.1% HSA or BSA and centrifuge at 200 x g for 10 minutes X 2
      8. The mononuclear cell preparation is ready.

      役に立ちましたか？

  2. What percentage of lymphocytes/monocytes, etc. would be expected if they use it on fresh human blood?

    1 回答

    1. When utilizing the Lymphocyte Separation Medium 1077 (cat. #C-44010) on fresh human whole blood, the expected percentage of lymphocytes/monocytes typical for this sample is usually in the range of 70-90% of the enriched PBMCs.

      役に立ちましたか？

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