The Mono-Poly resolving media has only a slight difference in formulation compared to Histopaque 11191, primarily in its density which can vary from 1.112 to 1.116 g/ml. In contrast, the specification for Histopaque 11191 is 1.119 g/ml +/- 0.001 g/ml.
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無菌性
sterile-filtered
フォーム
liquid
シェルフライフ
Expiry date on the label.
テクニック
cell culture | mammalian: suitable
不純物
endotoxin, tested
密度
1.119
アプリケーション
hematology
histology
保管温度
2-8°C
詳細
Histopaque®-1119は、滅菌済みで内毒素検査済みの、すぐに使用できる培地です。本溶液は、ポリスクロースおよびジアトリゾ酸ナトリウムからなり、密度が1.119 g/mL、pH 8.8～9.0に調整されています。
アプリケーション
Histopaque®-1119は、少量の全血または骨髄由来の生存単核細胞および顆粒球の迅速な回収を促進することから、Histopaque-1077との併用に適しています。
特徴および利点
Histopaque®-1119には、以下のような多くの長所があります：
- 生存細胞の最適な回収
- 血液細胞タイプの選択的分離が可能
- 細胞変形を防止
- 細胞生存能力を保存
- 外部からの細胞干渉がほとんどない
- 優れたバッチ間性能再現性
- 滅菌ろ過済み、およびエンドトキシン試験済み
- 遮光した場合に2～8°Cで少なくとも2年間安定
原理
この過程には、等容量のHistopaque-1077をHistopaque-1119の上に重ねることで二重勾配を形成することが必要です。抗凝固処理した全血を上側になっているHistopaque-1077培地上に注意深く重層します。遠心分離後、赤血球はポリスクロースによって凝集し、速やかに沈降します。顆粒球は下側のHistopaque-1077/1119界面に認められ、リンパ球やその他の単核細胞は上側の血漿/Histopaque-1077界面に認められます。上部界面に認められた大部分の外来性血小板は、洗浄段階で低速遠心分離により除去されます。赤血球による汚染はほとんど生じません。
法的情報
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
関連製品
製品番号
詳細
価格
シグナルワード
Warning
危険有害性情報
危険有害性の分類
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
保管分類コード
8A - Combustible corrosive hazardous materials
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
労働安全衛生法名称等を表示すべき危険物及び有害物
名称等を表示すべき危険物及び有害物
労働安全衛生法名称等を通知すべき危険物及び有害物
名称等を通知すべき危険物及び有害物
Jan Code
11191-BULK:
11191-100ML:4548173373478
11191-VAR:
11191-PROC:
11191-6X100ML:4548173373485
11191-PH:
11191PROC:
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Journal of bacteriology, 191(7), 2023-2032 (2008-12-31)
Group B Streptococcus (GBS) is major cause of invasive disease in newborn infants and the leading cause of neonatal meningitis. To gain access to the central nervous system (CNS), GBS must not only subvert host defenses in the bloodstream but
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資料
Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.
遠心分離は、沈降作用により粒子の分離を可能にします。遠心により粒子を分離する方法と、ストークスの法則を利用して沈降速度を計算する方法を説明します。
プロトコル
This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.
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Is there a product similar to Mono-Poly resolving media?
1 回答-
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Is the Histopaque-1119 solution compatible with blood stored in PAXgene cell-free DNA tubes for separating different cells from the blood when used in combination with Histopaque-1077 for a double gradient?
1 回答-
The PAX gene Blood DNA tubes were not tested for compatibility with any of the Histopaque products. As a general rule, fresh blood provides the best results with the Histopaque products, and it is generally not recommended to use blood stored for more than 24 hours.
役に立ちましたか？
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Is it possible to use Histopaque 1119 as a double gradient media with another brand of density gradient media, such as Ficoll paque plus, or does it only work with Histopaque 1077?
1 回答-
Histopaque and Ficoll Paque are chemically identical solutions. For all practical purposes, both have polysucrose 400.
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Are there any comparable products for Pancoll at densities 1.119 g/ml and 1.077 g/ml, to be used in cell isolation for gene expression studies or diagnostics.
1 回答-
As Pancoll is polysucrose-based, Histopaque and Ficoll Paque have essentially the same formulations. For Histopaque and Ficoll Paque, products are available with densities of 1.073, 1.077, 1.083, 1.084, and 1.119 g/ml. Any of the Histopaque and Ficoll Paque products with densities of 1.077 g/ml and 1.119 g/ml should be suitable crossmatches for Pancoll products with densities of 1.077 g/ml and 1.119 g/ml.
役に立ちましたか？
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Hi. I am trying to adapt an older protocol that calls for a certain percentage of Ficoll. What percentage does this product contain?
1 回答-
This product is a mixture of Ficoll (polysucrose 400) and sodium diatrizoate at 60 grams and 167 grams per liter, respectively. The solution may not be a suitable substitute for protocols requiring Ficoll, only. Please see product F5415 to review a 20% Ficoll solution in water. This item may a better option:
https://www.sigmaaldrich.com/product/sial/f5415Other pure Ficoll products are available. See these potential product numbers below:
F4375
F2637
F8016
F9378
F8636
F1418
GE17-0300-50
GE17-0300-10
GE17-0300-05役に立ちましたか？
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The Product 11191, Histopaque®-1119, froze in transit. Is the product still suitable for use?
1 回答-
Mix the bottles upon receipt several times to ensure the product is uniform. The products are shipped ambient and gradients in situ can form if the solution freezes during transport. As long as the bottle has not broken and no precipitate is noted, the product is suitable for use after slowly thawing and mixing.
役に立ちましたか？
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Can the procedure for using Product 11191, Histopaque®-1119, be used successfully with species such as rats and mice?
1 回答-
The Histopaque 1119-1 procedure was developed for use with human blood. Due to the lower number of circulating neutrophils in rat or mouse peripheral blood, the technique tends not to work as well with rats or mice.
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Can the procedure for Product 11191, Histopaque®-1119, be performed using the various Accuspin products?
1 回答-
No. Accuspin products are intended for use with just a single density gradient - such as Histopaque® 1077.
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When using Product 11191, Histopaque®-1119, what is the expected percent recovery for the band containing neutrophils.
1 回答-
No percent recovery data has been generated. Due to the importance of technique with the Histopaque 1119-1, percent recovery varies with the skill of the operator.
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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