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安全性情報

F5415

Sigma-Aldrich

Ficoll® 溶液

Type 400, 20% in H2O

別名:

Ready-to-use density gradient media

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25 ML
￥16,100
50 ML
￥26,600

￥16,100

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25 ML
￥16,100
50 ML
￥26,600

About This Item

CAS番号:
26873-85-8
MDL番号:
MFCD00081599
UNSPSCコード:
12352207
NACRES:
NA.75

￥16,100

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バルクの問い合わせ

無菌性

0.2 μm filtered

品質水準

200

タイプ

Type 400

フォーム

liquid

濃度

20% in H2O

テクニック

titration: suitable

色

clear colorless

溶解性

water: soluble

密度

1.055-1.075 g/mL

アプリケーション

hematology
histology

保管温度

2-8°C

関連するカテゴリー

詳細

Ficoll® 溶液は、高度分岐スクロースポリマーで、デキストランの多分散ミックスとして知られています。Ficoll®分子は、不活性で、近位尿細管により再吸収されません。 その構造は開放的で、変更可能であり、剛性の球状であるため、クラウディング実験で望ましい選択肢となります。“”
電気泳動およびハプテンキャリヤ-として使用されています。 密度勾配の調製に最も一般的に使われています。

アプリケーション

Ficoll® は、


  • 臍帯血単核細胞の単離[1]
  • CD133陰性細胞の単離[2]
  • 循環内皮前駆細胞の単離と増殖[3]

その他情報

ショ糖の非イオン性合成高分子

法的情報

Ficoll is a registered trademark of Cytiva

保管分類コード

12 - Non Combustible Liquids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

F5415-50ML:
F5415-25ML-PW:
F5415-25ML:
F5415-PH:
F5415-VAR:
F5415-50ML-PW:
F5415-BULK:
F5415-VAR-PW:

最新バージョンのいずれかを選択してください：

試験成績書（COA）

Lot/Batch Number
0000347993
0000347992
0000347993
SLCQ8241
SLCM9158

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Slide 1 of 1

1 of 1

Sigma-Aldrich

Sigma-Aldrich

H8889

Histopaque®-1077 Hybri-Max™

Junchao Xing et al.
Tissue engineering. Part C, Methods, 25(3), 137-147 (2019-02-09)
Patient-specific individual tissue-engineered bones (iTEBs) have been recognized as a promising strategy for treating large bone defects. However, current construction protocols of iTEBs vary between lots and lack standardization and quality control, hampering further research and application. This study was
Effect of mononuclear cells on morphometric of kidney parameters in hyperglycemic rat.
Salmani F, et al.
Annals of Experimental Biology, 2(3), 1-7 (2014)
Juliane Schmidt et al.
PloS one, 6(3), e18184-e18184 (2011-03-30)
The characterization of factors contributing to the formation and development of surface-associated bacterial communities known as biofilms has become an area of intense interest since biofilms have a major impact on human health, the environment and industry. Various studies have
Circulation enrichment of functional endothelial progenitor cells by infantile phototherapy.
Siavashi V, et al.
Journal of Cellular Biochemistry, 118(2), 330-340 (2017)
Xiaoliang Wu et al.
Shock (Augusta, Ga.), 51(6), 718-724 (2018-08-07)
The aim of this study was to investigate the correlation between plasma sphingosine-1-phosphate (S1P) and ceramide concentrations in sepsis, and the possible mechanisms for altered expression. Plasma S1P and ceramide concentrations were measured by HPLC-ESI-MS/MS. HLA-DR (human leukocyte antigen-DR) expression
関連する文献をすべて表示

プロトコル

Hematopoietic Stem Cell Culture Methods

Hematopoietic stem cell culture protocols for isolation, expansion, and differentiation of CD34+ cells including CFU assays.

質問

1–7/7 質問  

  1. What are the differences between Ficoll, Ficoll Paque Plus, Ficoll Paque Premium, Histopaque and the different products with different densities?

    1 回答

    1. 1. Ficoll-Paque PREMIUM products - Sterile, ready-to-use density gradient media for the preparation of mononuclear cells - Based on Ficoll-Paque PLUS - Used for isolating high yields of mononuclear cells from bone marrow, peripheral blood, and cord blood
      2. Regulatory Status - Neither Ficoll-Paque Plus nor Ficoll-Paque Premium products are approved for In Vitro Diagnostic Use - For clinical studies, a GMP grade product (Ficoll-Paque Premium) is preferred over a product produced under ISO 9001 - Both products are sterile and low in endotoxins
      3. Differences between Ficoll and Histopaque - Ficoll and Histopaque are essentially the same formulation - Ficoll is a trademark name for polysucrose 400, and Paque is a trademark name for sodium diatrizoate - Both can be used to separate various cell types based on different densities and species
      4.Cell Separation Using Ficoll and Histopaque - Different densities are used for different cell types and sometimes the same cell types, but from different species - Ficoll and Histopaque can be used to separate a variety of cell types based on different densities and species

      役に立ちましたか？

  2. What is the difference between Product F5415, Ficoll® solution 20%, and the polysucrose type 400 used in the Histopaque 1077-1?

    1 回答

    1. Ficoll is a registered trademark. The trademark was formerly held by Pharmacia, but today Amersham Biosciences/GE Healthcare hold the trademark.  Since Sigma Aldrich uses polysucrose type 400 in the preparation of the various Histopaque products, the name polysucrose 400 is utilized.

      役に立ちましたか？

  3. Is Product F5415, Ficoll® solution 20% suitable for the separation of mononuclear cells without further dilution?

    1 回答

    1. No.  The 20% solution of Ficoll has a density of approximately 1.066 to 1.068 g/mL.  Typically products such as Histopaque 1077-1 or Ficoll Paque have a density of approximately 1.076 to 1.078 g/mL.  Such ready to use solutions are also osmotically balanced by the addition of a lower molecular weight chemical.  Properly balanced solutions will typically have an osmolality in the range of approximately 280-300 mOsm/Kg. If a ready made product is required for the separation of mononuclear cells, products Histopaque 1077-1 or H8889 should be utilized.

      役に立ちましたか？

  4. Can more Ficoll be added to Product F5415, Ficoll® solution 20%, to increase the density to 1.077 g/mL?

    1 回答

    1. This is not recommended.  Ficoll has a molecular weight in the range of 400,000.  Even 20% solutions of such high molecular weight products have extremely low osmolality.  Without the addition of other lower molecular weight chemical, solutions of 20%+ of Ficoll are considered extremely hypotonic.  Cells exposed to hypotonic solutions would not maintain viability and are prone to bursting.

      役に立ちましたか？

  5. Is Product F5415, Ficoll® solution 20%, sterile?

    1 回答

    1. No claims are made for sterility for F5415.  If a sterile product is required for cell culture use,  product F8636 has been gamma irradiated and is considered a sterile product.  Product F8636 is a powder and does not come as a sterile solution.

      役に立ちましたか？

  6. Is there a Product Information Sheet for Product F5415, Ficoll® solution 20%?

    1 回答

    1. No.  However there is a Product Information Sheet for product F4375.  This product information sheet gives general information regarding Ficoll and possible uses.  If mononuclear cells are to be separated, a better choice of products is Histopaque 1077-1, H8889 or one of the prefilled Accuspin tubes.

      役に立ちましたか？

  7. What is the Department of Transportation shipping information for this product?

    1 回答

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      役に立ちましたか？

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