70794

BugBuster^® GST-Bind Purification Kit

Affinity purification of GST fusion proteins

• UNSPSCコード: 41106500
• NACRES: NA.32

特性

• メーカー／製品名: Novagen^®
• 保管条件: OK to freeze

詳細

詳細

Affinity purification of GST fusion proteins

The BugBuster^® GST•Bind<TMSYMBOL></TMSYMBOL> Purification Kit combines the GST•Bind Resin, GST•Bind Buffer Kit reagents and BugBuster Protein Extraction Reagent for convenient preparation of soluble cell extracts and affinity purification of GST•Tag<TMSYMBOL></TMSYMBOL> fusion proteins. BugBuster Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E. coli, resulting in the liberation of soluble protein. Cells are harvested by centrifugation as usual, followed by suspension in BugBuster Reagent. During a brief incubation, soluble proteins are released. The extract is clarified by centrifugation, which removes cell debris and insoluble proteins. The clarified extract is ready to apply to GST•Bind Resin.

構成

•2 x 100 mlBugBuster Protein Extraction Reagent

•10,000 UBenzonase Nuclease, purity >90%

•10 mlGST•Bind Resin

•pkg/4Chromatography Columns

•2 x 100 ml10X GST Bind/Wash Buffer

•40 ml10X Glutathione Reconstitution Buffer

•1 gGlutathione, Reduced

警告

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

法的情報

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• ピクトグラム: GHS02
• シグナルワード: Warning
• 危険有害性情報: H226
• 注意書き: P210 - P233 - P240 - P241 - P242 - P243
• 危険有害性の分類: Flam. Liq. 3
• 保管分類コード: 3 - Flammable liquids
• 引火点(°F): 96.8 °F
• 引火点(℃): 36 °C