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Merck
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安全性情報

70584-M

Millipore

BugBuster® Protein Extraction Reagent

別名:

Protein Extraction Reagent, Protein Extraction Solution

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About This Item

UNSPSCコード:
41106516
NACRES:
NA.32
価格・在庫情報は確認できません。詳細については、Merckをご覧ください。

フォーム

liquid

品質水準

100

メーカー／製品名

Novagen®

保管条件

OK to freeze
avoid repeated freeze/thaw cycles

保管温度

10-30°C

詳細

BugBuster® Protein Extraction Reagent is formulated for gentle disruption of Escherichia coli cell wall to liberate active proteins. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The proprietary formulation utilizes a tris-buffer-based mixture of non-ionic and zwitterionic detergents that is capable of cell wall perforation without denaturing the protein. BugBuster is also useful for the preparation of high-purity inclusion bodies for subsequent purification in instances where expressed proteins are insoluble.

アプリケーション

BugBuster® Protein Extraction Reagent has been used:
  • to lyse Escherichia coli BLR (DE3) cells for β-glucosidase (BglB) production and purification[1]
  • as a cell lysis buffer to treat the synechocystis PCC6803 transconjugants and measure the yellow fluorescent protein (YFP) fluorescence[2]
  • in lysis buffer for purification of glutathione S-transferase- heat shock protein family A (Hsp70) member 8 (GST- HSPA8)[3]

警告

Toxicity: Standard Handling (A)

法的情報

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

保管分類コード

12 - Non Combustible Liquids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

70584-4:
70584-5000ML:
70584-ML:
70584-1LPC:
70584-1000ML:
70584-3:

試験成績書（COA）

製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Patricia Armshaw et al.
Biotechnology for biofuels, 8, 201-201 (2015-12-03)
The use of photosynthetic autotrophs and in particular the model organism Synechocystis PCC6803 is receiving much attention for the production of sustainable biofuels and other economically useful products through metabolic engineering. Optimisation of metabolic-engineered organisms for high-level sustained production of
Dylan Alexander Carlin et al.
PloS one, 12(5), e0176255-e0176255 (2017-05-23)
Accurate modeling of enzyme activity and stability is an important goal of the protein engineering community. However, studies seeking to evaluate current progress are limited by small data sets of quantitative kinetic constants and thermal stability measurements. Here, we report
Tiejian Nie et al.
Autophagy, 17(10), 2923-2938 (2021-05-11)
As a highly dynamic organelle, mitochondria undergo constant fission and fusion to change their morphology and function, coping with various stress conditions. Loss of the balance between fission and fusion leads to impaired mitochondria function, which plays a critical role

資料

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

BugBuster® and Benzonase® Reagents are the Clear Solutions to Simple, Efficient Extraction of E. coli Proteins

This article shows the use of BugBuster® and Benzonase® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.

ReadyShield Phosphatase and Protease Inhibitor Cocktail FAQ

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

関連コンテンツ

Lysis & Protein Extraction

Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.

溶解・タンパク質抽出

細胞溶解およびタンパク質抽出法では、界面活性剤による可溶化から機械的破壊まで、さまざまな技術を概説し、研究ニーズをサポートします。

質問

1–3/3 質問  

  1. Can this be used to extract membrane proteins?

    1 回答

    1. BugBuster Protein Extraction Reagent is a mixture of non-ionic and zwitterionic detergents optimized for the extraction of soluble proteins. It does not solubilize insoluble proteins in inclusion bodies. BugBuster does not lyse cells but creates openings in the membrane to release proteins, similar to most detergent-based extractions. Traditionally, detergents are used to release membrane proteins into the soluble fraction, although not all membrane proteins will be released. Some may remain in the pellet and require denaturing conditions for release. Highly hydrophobic or membrane-associating proteins are likely to be found in the cell debris pellet. The extraction of membrane proteins using BugBuster has not been directly evaluated. Non-ionic detergents can solubilize some membrane proteins, but the extent to which BugBuster achieves this has not been analyzed. It is expected that some, but not all, membrane proteins would be solubilized using BugBuster, and therefore, its use for membrane protein extraction cannot be guaranteed.

      役に立ちましたか？

  2. can we use this for protein extarction of P aeruginosa bacteria for Carb NP test

    1 回答

    1. BugBuster® Protein Extraction Reagent is formulated for gentle disruption of E. coli cell wall. It is not tested for use with other organisms. Suitability would have to be determined experimentally.

      役に立ちましたか？

  3. Can I use bugbuster to lysis Mycobacterium tuberculosis(ATCC25177)?

    1 回答

    1. BugBuster® Protein Extraction Reagent is formulated for gentle disruption of E. coli cell wall. It is not tested for use with other organisms. Suitability would have to be determined experimentally.

      役に立ちましたか？

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