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詳細
The BugBuster® Master Mix enables efficient protein purification and other related applications. It replaces the need for mechanical methods like the French press or sonication. The two available package sizes contain sufficient reagents for extracting proteins from either 20 grams or 100 grams of cell paste.
アプリケーション
BugBuster® Master Mix has been used:
- to resuspend and lyse the cell pellets during protein purification
- to extract proteins for immunoblotting
- as a bacterial lysis buffer for the preparation of inclusion bodies
生物化学的／生理学的作用
BugBuster® Protein Extraction Reagent is specifically designed to gently disrupt the cell wall of E. coli, allowing the release of soluble proteins. This unique formulation utilizes a detergent mix that can effectively perforate the cell wall without denaturing the soluble proteins. By combining BugBuster Reagent, rLysozyme solution, and Benzonase Nuclease, our BugBuster Master Mix enhances protein extraction efficiency and simplifies downstream processing of protein extracts.
特徴および利点
- Efficient lysis of Gram-positive and Gram-negative bacteria
- Maximum recovery of active, soluble protein
- Capable of cell wall perforation without denaturing soluble protein
- Useful for the preparation of high purity inclusion bodies
- Compatible with protease and phosphatase inhibitor cocktail
- Offers a convenient, fast, and cost-effective solution for releasing the expressed target protein.
- The Master Mix eliminates the need to dilute or perform separate addition steps.
警告
Toxicity: Standard Handling (A)
法的情報
BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
保管分類コード
10 - Combustible liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
71456-25ML:
71456-3:
71456-EA:
71456-ML:
71456-4:
71456-100ML:
71456-1L:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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資料
Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens
関連コンテンツ
界面活性剤可溶化、凍結融解溶解、浸透圧ショック、超音波処理、酵素細胞溶解、および、機械的破砕技術（ダウンス、ポリトロン、乳鉢と乳棒によるホモジナイズなど）を含む細胞溶解とタンパク質抽出法の概要。
Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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