07-442

Anti-trimethyl-Histone H3 (Lys9) Antibody

Upstate^®, from rabbit

• 別名: H3K9me3, Histone H3 (tri methyl K9), H3 histone family, member T, histone 3, H3, histone cluster 3, H3
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 交差性: human, mouse, rat, chicken
• メーカー／製品名: Upstate^®
• テクニック: ChIP: suitable (ChIP-seq); dot blot: suitable; immunocytochemistry: suitable; inhibition assay: suitable (peptide); multiplexing: suitable; western blot: suitable
• アイソタイプ: IgG
• NCBIアクセッション番号: NM_003493
• UniProtアクセッション番号: Q16695
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: trimethylation (Lys9)
• 遺伝子情報: human ... H3C1(8350) ; mouse ... H3C1(360198) ; rat ... H3C1(679994)

詳細

詳細

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

特異性

Expected to cross-react with chicken, mouse and rat.

Trimethyl-histone H3 (Lys9). Broad species cross-reactivity expected.

免疫原

Epitope: Lys9

KLH-conjugated, 2X-branched synthetic peptide containing the sequence …AR[me3K]ST… in which me3K corresponds to trimethyl-lysine at residue 9 of human histone H3.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
ヒストン

Anti-trimethyl-Histone H3 (Lys9), Cat. No. 07-442 is a rabbit polyclonal antibody that detects Histone H3 (Met3-Lys9) and is tested for use in Western Blotting, Dot Blot, Peptide Inhibition Assay, and Immunocytochemistry.

Tested Applications

Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected Histone H3 (Met3-Lys9) in Mouse Embryo Fibroblasts (MEF).

Peptide Inhibition Assay: Target band detection in acid extract of HeLa cells was prevented by pre-blocking of a representative lot with the immunogen Histone H3 (Met3-Lys9) peptide, but not the corresponding unmodified Histone H3 peptide.

Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected trimethyl-Histone H3 (Lys9) peptide, but did not detect di-methyl Histone H3 (Lys9) peptide, mono-methyl Histone H3 (Lys9) peptide, nor unmodified Histone H3 (1-19) peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

品質

Evaluated by Western Blotting in HeLa cell acid extract.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Histone H3-Lys9me3 in HeLa cell acid extract, but did not detect unmodified recombinant Histone H3.

ターゲットの説明

17 kDa

物理的形状

Protein A chromatography

Format: Purified

Purified rabbit polyclonal IgG in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol.

保管および安定性

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

アナリシスノート

Control
Acid-extracted proteins from HeLa cells.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1