Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis Express column efficiency. The suggested starting point for flow rate for Ascentis Express columns: 1.6 mL/min for 4.6 mm ID; 0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.
53838-U
Ascentis® Express 90Å C8 (2.7 μm) HPLC Columns
L × I.D. 15 cm × 4.6 mm, HPLC Column
Sinonimo/i:
Colonna per HPLC Core-shell (SPP) Fused Core C8
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About This Item
Codice UNSPSC:
41115700
eCl@ss:
32110501
NACRES:
SB.52
893,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Prodotti consigliati
Nome del prodotto
Colonna HPLC Ascentis® Express C8, 2,7 μm, 2.7 μm particle size, L × I.D. 15 cm × 4.6 mm
Materiali
stainless steel column
Livello qualitativo
agenzia
suitable for USP L7
Nome Commerciale
Ascentis®
Caratteristiche
endcapped
Produttore/marchio commerciale
Ascentis®
Confezionamento
1 ea of
Parametri
60 °C temp. range
600 bar max. pressure (9000 psi)
tecniche
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
Lungh. × D.I.
15 cm × 4.6 mm
Area superficiale
135 m2/g
Impurezze
<5 ppm metals
Matrice
Fused-Core particle platform
superficially porous particle
Gruppo funzionale matrice
C8 (octyl) phase
Dimensione particelle
2.7 μm
Dimensione pori
90 Å
Intervallo di pH d’impiego
2-9
applicazioni
food and beverages
Tecnica di separazione
reversed phase
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Categorie correlate
Descrizione generale
Colonne HPLC Ascentis Express, attraverso l′uso della tecnologia di particelle Fused-Core® offrono l′alta velocità ed efficienza delle particelle sotto 2 μm nel contempo mantenendo contropressioni più basse. La combinazione di alta efficienza e bassa contropressione porta vantaggi agli utenti di UPLC® (o altri sistemi UHPLC a pressione ultra-alta), nonché agli utenti di HPLC convenzionali.
Andare alla pagina Ascentis Express per ulteriori informazioni sulla nuova zecnologia presente in questa colonna.
Andare alla pagina Ascentis Express per ulteriori informazioni sulla nuova zecnologia presente in questa colonna.
Applicazioni
- Tendenze recenti nell'HPLC ultraveloce: colonne di silice superficialmente porosa di nuova generazione. Questa review esplora l'evoluzione e le prestazioni delle colonne di silice superficialmente porosa nella cromatografia liquida ad alte prestazioni (HPLC), comprese quelle simili alla colonna Ascentis Express C8, da 2,7 μm. La rassegna si concentra in particolare sull'impatto della tecnologia sul miglioramento della velocità e dell'efficienza analitiche (Ali et al., 2012).
- Sviluppo e validazione di un metodo HPLC-MS/MS per la determinazione del clopidogrel nel plasma umano. Uso di campioni "incurred” per testare la retroconversione. Questo studio illustra lo sviluppo di un metodo analitico HPLC-MS/MS che utilizza colonne come la Ascentis Express C8, 2,7 μm, per quantificare il clopidogrel nel plasma, dimostrando l'utilità della colonna nelle valutazioni farmacocinetiche cliniche (Silvestro et al., 2010).
Note legali
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
UPLC is a registered trademark of Waters
Precolonna
N° Catalogo
Descrizione
Determinazione del prezzo
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Richiesto ma non fornito
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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Articoli
When a C18 doesn′t give the desired separation, or your sample contains compounds that are known to be difficult to retain or resolve on a C18, consider changing to an Ascentis® Express C8 column.
Protocolli
-methylcarbamate 10 μg/mL; Diuron; Propham; Siduron; Methiocarb, analytical standard; Linuron 10 μg/mL; Swep 10 μg/mL; Chlorpropham 10 μg/mL; Barban; Neburon
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What flow rate should I use with Ascentis® Express HPLC Columns?
1 risposta-
Utile?
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Can Ascentis® Express HPLC Columns be used for LC-MS?
1 risposta-
Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis Express columns for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.
Utile?
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Can I use Ascentis Express on a UHPLC system?
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Yes. Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments. In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
Utile?
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Do I need special fittings and tubing to connect Ascentis® Express HPLC Columns?
1 risposta-
While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.
Utile?
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Is there anything special I need to do to my HPLC system to use Ascentis Express?
1 risposta-
Nothing special is required to use Ascentis Express HPLC columns. To obtain the full benefits of Ascentis Express, one should minimize dispersion in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Systems for Ascentis Express Columns (T407102).
Utile?
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What is the Department of Transportation shipping information for this product?
1 risposta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Utile?
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Can I use Ascentis Express on any type of HPLC system?
1 risposta-
Ascentis Express HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems. Columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.
Utile?
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How can I measure my instrument bandwidth (IBW) and determine what Ascentis® Express HPLC Columns can be used with minimal efficiency loss created by too much internal instrument volume?
1 risposta-
The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.
Utile?
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