Product 53301-U Ascentis® Express Peptide ES C18 HPLC Column HPLC columns is capable of use on standard HPLC systems as well as UHPLC systems. This column is packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.
53301-U
Ascentis® Express Peptide 160 Å ES-C18 (2.7 μm) HPLC Columns
L × I.D. 5 cm × 2.1 mm, HPLC Column
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About This Item
Codice UNSPSC:
41115700
eCl@ss:
32110501
NACRES:
SB.52
569,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Prodotti consigliati
Nome del prodotto
Colonna HPLC Ascentis® peptidi Express ES- C18, 2,7 μm, 2.7 μm particle size, L × I.D. 5 cm × 2.1 mm
Materiali
stainless steel column
Livello qualitativo
agenzia
suitable for USP L1
Nome Commerciale
Ascentis®
Caratteristiche
endcapped: no
Produttore/marchio commerciale
Ascentis®
Confezionamento
1 ea of
Parametri
≤100 °C temp. range
600 bar max. pressure (9000 psi)
tecniche
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
Lungh. × D.I.
5 cm × 2.1 mm
Area superficiale
90 m2/g
Impurezze
<5 ppm metals
Matrice
Fused-Core particle platform
superficially porous particle
Gruppo funzionale matrice
C18 (octadecyl) phase
Dimensione particelle
2.7 μm
Dimensione pori
160 Å
Intervallo di pH d’impiego
1-9
Tecnica di separazione
reversed phase
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Descrizione generale
Le colonne Ascentis ES-C18 per peptidi espressi sono concepite specificamente per la separazione di composti a più alto peso molecolare come peptidi e piccole proteine. Queste colonne contengono particelle in tecnologia avanzata Fused-Core che hanno pori più larghi (160 Å contro i 90 Å di una colonna Ascentis Express standard), legate con ligandi C18 protetti stericamente per garantire extra stabilità (ES) a pH molto basso (< 1) e alte temperature (fino a 100 ºC). Questo espande notevolmente la gamma di applicazione per le colonne Ascentis Express.
Note legali
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
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Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Meiyun Shi et al.
Journal of separation science, 38(8), 1351-1357 (2015-01-30)
The pentapeptide thymopentin (Arg-Lys-Asp-Val-Tyr, RKDVY) corresponds to amino acids 32-36 of the 49 amino acid immunomodulatory polypeptide, thymopoietin, whose biological activity is partially reproduced. Thymopentin is widely used in the clinic and represents a promising target for drug design but
E Lesellier
Journal of chromatography. A, 1266, 34-42 (2012-11-03)
The recent introduction of new stationary phases for liquid chromatography based on superficially porous particles, called core-shell or fused-core, dramatically improved the separation performances through very high efficiency, due mainly to reduced eddy diffusion. However, few studies have evaluated the
C Gröer et al.
Journal of pharmaceutical and biomedical analysis, 100, 393-401 (2014-09-15)
Cytochrome P450 (CYP) enzymes and UDP-glucuronosyltransferases (UGT) are major determinants in the pharmacokinetics of most drugs on the market. To investigate their impact on intestinal and hepatic drug metabolism, we developed and validated quantification methods for nine CYP (CYP1A2, CYP2B6
Articoli
Choose Ascentis® Express Peptide ES-C18 U/HPLC Columns based on Fused-Core® technology for fast and efficient separation of high-molecular weight compounds, such as peptides and small proteins. With a 2.7 µm particle size and rigorous testing, these columns offer reliable results and high efficiency.
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Can I use product 53301-U Ascentis® Express Peptide ES C18 HPLC Column on any type of HPLC system?
1 risposta-
Utile?
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Can I use product 53301-U Ascentis® Express Peptide ES C18 HPLC Column on a UHPLC system?
1 risposta-
Yes. product 53301-U Ascentis® Express Peptide ES C18 HPLC columns are packed in a way making them suitable for these ultra high pressure instruments. In fact, product 53301-U Ascentis® Express Peptide ES C18 HPLC Column outperforms sub-2 μm micron columns on many applications since product 53301-U Ascentis® Express Peptide ES C18 HPLC Column provides the benefits of sub-2 μm particles but at much lower back pressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
Utile?
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Are guard columns available for product 53301-U Ascentis® Express Peptide ES C18 HPLC Column?
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Yes, we have guard columns available for product 53301-U Ascentis® Express Peptide ES C18 HPLC Column. Product 53500-U is the Ascentis® Express Guard Holder and Product 53536-U Ascentis® Express Peptide ES C18 Guard Cartridge is a package of three guard cartridges, 2.7 μm particle size, L 5mm × I.D. 2.1 mm .
Utile?
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What is the pressure rating for product 53301-U Ascentis® Express Peptide ES C18 HPLC Column?
1 risposta-
This product 53301-U Ascentis® Express Peptide ES C18 HPLC Column is stable to operating pressures up to 600 bar (9000 psi)
Utile?
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What is the carbon load for product 53301-U Ascentis® Express Peptide ES C18 HPLC Column?
1 risposta-
Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles. The carbon coverage for product 53301-U Ascentis® Express Peptide ES C18 HPLC column is 2 micromoles/meter-squared.
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What flow rate should I use with product 53301-U Ascentis® Express Peptide ES C18 HPLC columns?
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Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize product 53301-U Ascentis® Express Peptide ES C18 HPLC column efficiency. The suggested starting point for flow rate for Ascentis® Express columns: 1.6 mL/min for 4.6 mm ID; 0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.
Utile?
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Do I need special fittings and tubing to connect product 53301-U Ascentis® Express Peptide ES C18 HPLC ColumnExpress columns?
1 risposta-
While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.
Utile?
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How can I measure my instrument bandwidth (IBW) and determine what columns can be used with minimal efficiency loss created by too much internal instrument volume?
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The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.
Utile?
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Can product 53301-U Ascentis® Express Peptide ES C18 HPLC column be used for LC-MS?
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Yes, Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of product 53301-U Ascentis® Express Peptide ES C18 HPLC Column for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.
Utile?
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What is the Department of Transportation shipping information for this product?
1 risposta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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