T5425

Transforming Growth Factor-β3 human

≥97% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, powder, suitable for cell culture

• Sinonimo/i: hTGF-β3, TGF-β3
• Numero MDL: MFCD00213054
• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• Nome del prodotto: Transforming Growth Factor-β3 human, TGF-β3, recombinant, expressed in baculovirus infected Sf21 cells, powder, suitable for cell culture
• Origine biologica: human
• Livello qualitativo: 100
• Ricombinante: expressed in baculovirus infected Sf21 cells
• Saggio: ≥97% (SDS-PAGE)
• Stato: powder
• Potenza: 0.01-0.09 ng/mL ED₅₀/EC₅₀
• Qualità: endotoxin tested
• PM: predicted mol wt ~25 kDa
• Confezionamento: pkg of 2 μg
• Condizioni di stoccaggio: avoid repeated freeze/thaw cycles
• tecniche: cell culture | mammalian: suitable
• Impurezze: <0.01 EU/μg
• N° accesso UniProt: P10600
• Temperatura di conservazione: −20°C
• Informazioni sul gene: human ... TGFB3(7043)

Descrizione

Descrizione generale

The TGFβ3 (transforming Growth Factor-β3) gene is mapped to human chromosome 14q24.3.[1]

Applicazioni

Transforming Growth Factor-β3 human has been used to study the role of TGF-β3 in healing tendon-to-bone insertion and in chondrogenic differentiation.[2][3]

Azioni biochim/fisiol

TGF-β (transforming growth factor-β3) isoforms are significantly involved in inducing inflammatory responses. It is associated with vascular diseases, wound healing and ameliorating effect in venous leg ulcer. TGF-β3 has an antagonistic effect on TGF-β1 and reduces the degree of collagen type I deposition and fibroblast differentiation. As a result TGF-β3 might lead to ulcer chronicity due to hindered myofibroblasts formation.[4] TGF-β is a key player in tumor formation and progression. Increased TGF-β3 expression is observed in cancer and adipose tissue fibrosis.[5][6] TGF-β3 is crucial in tissue regeneration and scarless tissue repair.[7]

TGF-β3 is less prevalent in natural expression than either TGF-β1 or TGF-β2, but it is the most abundant mRNA expressed in chick embryos. It is also expressed in human umbilical cord, in a variety of mesenchymal cells of human and rodent origin, and in several human carcinoma cells.

Stato fisico

Lyophilized from a 0.2 μm filtered solution in 35% acetonitrile and 0.1% trifluoroacetic acid containing 50 μg bovine serum albumin per 1 μg of cytokine.

Risultati analitici

The biological activity is measured by its ability to inhibit the IL-4-dependent proliferation of mouse HT-2 cells.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)