H8541

Fattore di crescita trasformante-β1

≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

• Sinonimo/i: TGF-β1
• Numero MDL: MFCD00166089
• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• Nome del prodotto: Fattore di crescita trasformante-β1, TGF-β1, Xeno-free, recombinant, expressed in HEK 293 cells, suitable for cell culture
• Origine biologica: human
• Livello qualitativo: 300
• Ricombinante: expressed in HEK 293 cells
• Saggio: ≥95% (SDS-PAGE)
• Stato: lyophilized powder
• Potenza: ≤0.5 ng/mL EC₅₀
• Qualità: endotoxin tested
• PM: dimer 25 kDa (non-glycosylated)
• Confezionamento: pkg of 5 μg
• Condizioni di stoccaggio: avoid repeated freeze/thaw cycles
• tecniche: cell culture | mammalian: suitable
• Impurezze: ≤1 EU/μg
• N° accesso UniProt: P01137
• Temperatura di conservazione: -10 to -25°C
• Informazioni sul gene: human ... TGFB1(7040)

Descrizione

Descrizione generale

The TGFB1 gene is mapped to human chromosome 19q13.2 and codes for a 25 kDa peptide.[1][2] TGF-ß1 polypeptide is produced as a precursor with hydrophobic signal sequence, pro-region and mature peptide. Cleavage of the precursor at tetrabasic cleavage site results in monomers of carboxy-terminal 112 amino acids. The biologically active form of Tgfb1 exists as a disulphide-linked homodimer.[3]

Applicazioni

Transforming Growth Factor-β1 (TGF-β) human has been used :

• to study the expression of PDGFRB (Platelet derived growth factor receptor β) in association with transforming growth factor-β signaling.[4]
• to stimulate lung fibrosis in human fetal lung fibroblast cell line in order to further measure the in-plane elasticity of live cell layers using a pressure sensor embedded microfluidic device.[5]
• to study the effect of TGF-β on HTRA1 (high-temperature requirement A1)-affected Wnt target genes.[6]

Azioni biochim/fisiol

Il fattore di crescita trasformante-β1 (TGF-β1) è prodotto da parecchi tipi cellulari, ma risulta essere massimamente concentrato nelle piastrine dei mammiferi, ove è presente a livelli all′incirca quadrupli rispetto al TGF-β2.

TGF-β1 is a multifunctional peptide capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types.[2] Transformed as well as non-neoplastic tissues release transforming growth factors and essentially all cells possess a specific TGF-β1 receptor. In general, cells of mesenchymal origin appear to be stimulated by TGF-β1; whereas, hepatocytes, T and B lymphocytes, keratinocytes, and many epithelial cells are inhibited by the peptide. TGF-β1 interacts with Epidermal Growth Factor (EGF), Platelet Derived Growth Factor (PDGF), Fibroblast Growth Factor (FGF), and T Cell Growth Factor (T-CGF) either by enhancing or antagonizing their characteristic actions. TGF-β1 plays a fundamental role in tissue growth and differentiation by involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation, and immune cell function.[7] TGF-β1 is associated with the developmental and metastasis of colorectal cancer.[8]

Stato fisico

Lyophilized from a 0.2 μm filtered solution of 50 mM NaOAc pH 4.5

Risultati analitici

The activity was determined by the dose dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2).

Informazioni sulla sicurezza

• Pittogrammi: GHS05
• Avvertenze: Danger
• Indicazioni di pericolo: H314
• Consigli di prudenza: P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
• Classi di pericolo: Eye Dam. 1 - Skin Corr. 1B
• Codice della classe di stoccaggio: 8A - Combustible corrosive hazardous materials
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable