T4427
Terminal Transferase from calf thymus
buffered aqueous glycerol solution
Sinonimo/i:
TdT, Terminal Deoxynucleotidyl Transferase
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About This Item
Prodotti consigliati
Grado
for molecular biology
Stato
buffered aqueous glycerol solution
PM
60 kDa
Concentrazione
>5000 U/mL
N° accesso UniProt
Attività estranea
Exonuclease and endonuclease, free
Temperatura di conservazione
−20°C
Informazioni sul gene
cow ... DNTT(281120)
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Descrizione generale
Bovine terminal transferase (TdT) is a primer-dependent polymerase that catalyzes the addition of deoxynucleotides to the 3′-OH terminus of DNA molecules with the release of inorganic phosphate. TdT reacts preferentially with either single-stranded DNA molecules or double-stranded-DNA with 3′ overhangs, but procedures have been developed to label blunt ends or 3′-recessive ends. In a reaction mixture, the divalent ion (Co2+, Mn2+, Mg2+) will influence purine and pyrimidine polymerization rate. Activities of TdT are also affected by the bases (dATP, dCTP, dGTP and dTTP) present.
Applicazioni
Suitable for:
- Addition of homopolymers to vectors, inserts and cDNA for cloning
- Labeling the 3′-end of double- and single-stranded DNA with non-radioactive or radioactive labels
- Carrying out in vitro mutagenesis by adding single nucleotides to DNA
- Use in TUNEL assays
Componenti
Terminal transferase is supplied as a solution of 50 mM potassium phosphate (pH 7.4), 1 mM 2-mercaptoethanol and 50% glycerol (v/v).
Definizione di unità
One unit will incorporate 1 nanomole of dATP into acid-precipitable material in one hour at 37 °C using d(pT)6 as primer.
Risultati analitici
Activity assay uses 200 mM potassium cacodylate, pH 7.2, 4 mM MgCl2, 1 mM 2-mercaptoethanol, 1 mM 3H-dATP, 70 μM d(pT)6, 37 °C.
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Resp. Sens. 1
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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D G Remick et al.
The American journal of pathology, 136(1), 49-60 (1990-01-01)
Tumor necrosis factor-alpha (TNF) has been implicated strongly as a principal mediator in the pathogenesis of septic shock. The authors investigated the in vivo production of TNF in CBA/J and CD-1 mice that had been primed by an intraperitoneal injection
Edward A Motea et al.
ACS chemical biology, 7(6), 988-998 (2012-03-07)
Acute lymphoblastic leukemia (ALL) is the most common type of childhood cancer, presenting with approximately 5,000 new cases each year in the United States. An interesting enzyme implicated in this disease is terminal deoxynucleotidyl transferase (TdT), a specialized DNA polymerase
U Weiland et al.
Cardiovascular research, 45(3), 671-678 (2000-03-23)
Apoptosis of cardiomyocytes may contribute to ischemia-reperfusion injury. The role of nitric oxide (NO) in apoptosis is controversial. Therefore, we investigated the effect of NO synthase inhibition on apoptosis of cardiomyocytes during ischemia and reperfusion and elucidated the underlying mechanisms.
Tadashi Terada
International journal of clinical and experimental pathology, 5(2), 167-170 (2012-03-09)
Although the definition of precursor T lymphoblastic lymphoma (T-LBL) is based only on histopathology, most cases cannot be diagnosed only by HE sections. Since 95% of T-LBL expresses TdT and TdT is expressed only in lymphoblasts, immunohistochemical demonstration of TdT
[Thymoma with extensive coagulation necrosis: report of two cases].
Jian Li et al.
Zhonghua bing li xue za zhi = Chinese journal of pathology, 41(2), 132-133 (2012-03-30)
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