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T3038

Sigma-Aldrich

Trizma® hydrochloride solution

1 M, BioReagent, for molecular biology

Sinonimo/i:

Tris hydrochloride solution

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1 L
126,00 €

126,00 €


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1 L
126,00 €

About This Item

Numero MDL:
Codice UNSPSC:
12161700
ID PubChem:
NACRES:
NA.25

126,00 €


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Grado

for molecular biology
for molecular biology

Livello qualitativo

Sterilità

0.2 μm filtered

Nome Commerciale

BioReagent

Stato

solution

Concentrazione

1 M

Impurezze

DNase, RNase, NICKase and protease, none detected

pH

8.0

applicazioni

agriculture

Stringa SMILE

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
QKNYBSVHEMOAJP-UHFFFAOYSA-N

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Descrizione generale

Trizma® hydrochloride solution is a useful biological buffer.

Applicazioni

Trizma® hydrochloride solution may be used in the following studies:
  • As buffer for the 2-D electrophoresis of rat fibroblast cell.[1]
  • As buffer for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length).[2]
  • Selective immunoprecipitation of biotin-labeled DNA with antibiotin IgG and Staphylococcus sp.[3]
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Altre note

Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.

Note legali

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


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Certificati d'analisi (COA)

Lot/Batch Number

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M G Murray et al.
Nucleic acids research, 8(19), 4321-4325 (1980-10-10)
A method is presented for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length) which is free of contaminants which interfere with complete digestion by restriction endonucleases. The procedure yields total cellular DNA
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Proceedings of the National Academy of Sciences of the United States of America, 78(11), 6633-6637 (1981-11-01)
Analogs of dUTP and UTP that contain a biotin molecule covalently bound to the C-5 position of the pyrimidine ring through an allylamine linker arm have been synthesized. These biotin-labeled nucleotides are efficient substrates for a variety of DNA and
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Domande

  1. What is the tris molarity in Trizma hydrochloride buffer cat# 41573 ?

    1 risposta
    1. Trizma is a registered trademark. The concentration of this product is between 0.95 - 1.05 M. The exact concentration is listed on the lot specific Certificate of Analysis: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/T3038-1LSLCQ0820.pdf.

      Utile?

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