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T2694

Sigma-Aldrich

Trizma® hydrochloride solution

pH 8.0, BioPerformance Certified, 1 M, suitable for cell culture

Sinonimo/i:

Tris hydrochloride solution

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100 ML
45,30 €
1 L
109,00 €

45,30 €

Prezzo di listino53,30 €Risparmia il 15%

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Cambia visualizzazione
100 ML
45,30 €
1 L
109,00 €

About This Item

Codice UNSPSC:
12161700
NACRES:
NA.25

45,30 €

Prezzo di listino53,30 €Risparmia il 15%

Per informazioni sulla disponibilità, contatta il Servizio Clienti.

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Grado

BioPerformance Certified
Molecular Biology
for molecular biology

Livello qualitativo

Stato

solution

Concentrazione

1 M

tecniche

cell culture | mammalian: suitable

Impurezze

DNase, RNase, Protease, none detected
bioburden, tested
endotoxin, tested
≤5 ppm Heavy metals (as Pb)

pH

8.0

Intervallo di pH utile

7.0-9.0

Assorbimento

≤0.05 at 290 at 40%

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Descrizione generale

A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Applicazioni

Trizma® hydrochloride solution has been used:
  • as a component of extraction buffer for Plasmodiophora brassicae DNA isolation[1]
  • as a component of resuspension buffer for human genomic DNA fragmented sample[2]
  • for adjusting pH of plasma samples and as a component of reconstitution and neutralization buffer[3]

Note legali

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


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Certificati d'analisi (COA)

Lot/Batch Number

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Olivier Harismendy et al.
Genome biology, 12(12), R124-R124 (2011-12-22)
Ultra-deep targeted sequencing (UDT-Seq) can identify subclonal somatic mutations in tumor samples. Early assays' limited breadth and depth restrict their clinical utility. Here, we target 71 kb of mutational hotspots in 42 cancer genes. We present novel methods enhancing both
Anti-peptide monoclonal antibodies generated for immuno-MRM assays have a high probability of supporting Western blot and ELISA
Schoenherr RM, et al.
Molecular and Cellular Proteomics, mcp-O114 (2014)
Accurate variant detection across non-amplified and whole genome amplified DNA using targeted next generation sequencing
ElSharawy A, et al.
BMC Genomics, 13(1), 500-500 (2012)
Root Gall Formation, Resting Spore Isolation and High Molecular Weight DNA Extraction of Plasmodiophora brassicae
Mehrabi S, et al.
Bio-protocol (2018)
Nishant Mehta et al.
Cell reports, 28(10), 2509-2516 (2019-09-05)
V-domain immunoglobulin (Ig) suppressor of T cell activation (VISTA) is an immune checkpoint protein that inhibits the T cell response against cancer. Similar to PD-1 and CTLA-4, a blockade of VISTA promotes tumor clearance by the immune system. Here, we report a

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  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 risposta
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 risposta
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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