For column chromatography, no more than 20 mM is suggested in the extract, equilibration, and wash buffers to prevent non-specific binding of proteins. No more than 250 mM is suggested for the elution buffers. Many proteins will elute with imidazole levels as low as 100 to 200 mM. For batch methods the imidazole concentration may have to be reduced or eliminated.When a protein is expressed at low levels, the presence of the imidazole limits the binding of the protein in the batch method but not when used in a column.
P6611
HIS-Select® Nickel Affinity Gel
HIS-Select® Nickel Affinity Gel
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(1:1 suspension in a 20% ethanol solution)
Sinonimo/i:
Ni-NTA resin, nickel charged agarose
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5 ML
146,00 €
25 ML
473,00 €
100 ML
1.460,00 €
500 ML
6.570,00 €
146,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Scegli un formato
Cambia visualizzazione
5 ML
146,00 €
25 ML
473,00 €
100 ML
1.460,00 €
500 ML
6.570,00 €
About This Item
Codice UNSPSC:
12352200
NACRES:
NA.56
146,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Prodotti consigliati
Coniugato
magnetic beads
Livello qualitativo
Stato
(1:1 suspension in a 20% ethanol solution)
Caratteristiche
hydrophilic
Confezionamento
pkg of 1 mL
pkg of 100 mL
pkg of 25 mL
pkg of 5 mL
pkg of 500 mL
Concentrazione
1.5-2.4 mL/mL (suspension in packed gel)
tecniche
protein purification: suitable
Colore
faint blue to very dark blue
Matrice
6% Beaded Agarose
Capacità
>15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)
Temp. transizione
flash point 32 °C (closed cup)
Temperatura di conservazione
2-8°C
Descrizione generale
HIS-Select® Nickel Affinity Gel is an immobilized metal ion affinity chromatography (IMAC) product, used for the purification of His-tagged proteins. While the unique, non-charged, hydrophilic linkage of the proprietary quadridentate NTA chelate group to the beaded agarose charged with nickel ensures high selectivity for small to medium scale His-tag protein purification, it also results in reduced non-specific binding of other proteins. HIS-Select Nickel Affinity Gel is selective for recombinant proteins with His-tags and exhibits low non-specific binding of other proteins. The selectivity can be modulated with the inclusion of imidazole during chromatography.
HIS-Select® Nickel Affinity Gel is durable and can capture the recombinant proteins with histidine tags at a high flow rate. Recombinant proteins with histidine tags are bound using either native or denaturing conditions.
Applicazioni
Caratteristiche e vantaggi
- High selectivity for higher purity.
- Unique non-charged hydrophilic linkage reduces non-specific binding.
- Binding capacity for histidine-tagged protein is greater than 15 mg/mL.
- Binding under denaturing or non-denaturing conditions.
- One-step purification.
- Minimizes unwanted ionic interactions.
- Minimal nickel leaching.
- Bead size: 45-165 μm.
Linkage
It is also available with the EZview™ technology (Product Code E3528).
Stato fisico
1:1 suspension in a 20% ethanol solution
Stoccaggio e stabilità
HIS-Select Nickel Affinity Gel is stable for at least one year when stored properly. The HIS-Select Nickel Affinity Gel should be cleaned after each use and an antimicrobial agent such as 20% ethanol should be added to the storage buffer.
Note legali
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Flam. Liq. 3
Codice della classe di stoccaggio
3 - Flammable liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
89.6 °F - closed cup
Punto d’infiammabilità (°C)
32 °C - closed cup
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Contenuto correlato
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Can imidazole be used with HIS-Select® Nickel Affinity Gel, Product P6611?
1 risposta-
Utile?
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Why won't my recombinant protein with a histidine-containing tag bind to HIS-Select® Nickel Affinity Gel, Product P6611?
1 risposta-
Verify the pH and composition of sample and equilibration buffers. Make sure there are no chelating or reducing agents present in the extraction buffer. If using the batch mode, remove imidazole. Run the affinity purification under denaturing conditions. Run a Western blot of the extract to verify that the recombinant protein is present.
Utile?
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Can I use SDS with HIS-Select® Nickel Affinity Gel, Product P6611?
1 risposta-
0.1% SDS has been used with some samples, with no adverse effects on the observed protein binding. However, SDS will effectively coat proteins and may block the binding to the column. It is probably very protein-specific and an SDS concentration that works for one protein may not work for another.
Utile?
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What needs to be done if the HIS-Select® Nickel Affinity Gel, Product P6611, resin turns brown on reuse?
1 risposta-
During purification many protein extracts tend to discolor an affinity gel during the loading step. The original color will return after the wash or elution step. If the color is still not changing strip and recharge the affinity gel with nickel. Wash with EDTA and recharge with Nickel solution.
Utile?
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What is the Department of Transportation shipping information for this product?
1 risposta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Utile?
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Can Tris buffers be used instead of phosphate buffer for HIS-Select® Nickel Affinity Gel, Product P6611?
1 risposta-
Yes, Tris buffers should work.
Utile?
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