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H0537

Millipore

HIS-Select® HF Nickel Affinity Gel

Sinonimo/i:

Ni-NTA HF resin

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About This Item

Codice UNSPSC:
41106500
NACRES:
NA.56

Forma fisica

suspension

Livello qualitativo

200

tecniche

affinity chromatography: suitable

Matrice

Highly cross-linked 6% Beaded Agarose

Capacità

15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)

Temperatura di conservazione

2-8°C

Descrizione generale

HIS-Select High Flow Nickel Affinity Gel is an immobilized metal-ion affinity chromatography (IMAC) gel that is designed to specifically bind His-tag proteins (histidine containing proteins) in chromatographic systems with pressures up to 200 psi and a maximum linear flow rate of 3,000 cm/hr.

Applicazioni

Affinity gel is suitable for the purification of histidine tagged proteins (His-tag proteins) in native and denaturing conditions.
Nickel Affinity Gel for the purification of His-tag® proteins (histidine tagged proteins). HIS-Select® High Flow (HF) brings the superior selectivity of HIS-Select technology to a highly cross-linked agarose for higher flow rates and mechanical stability under pressure. HIS-Select HF is designed for production scale purification and FPLC™ applications. As with other HIS-Select products, the non-charged, hydrophilic linkage of the nickel-nitrilotriacetic acid (Ni-NTA) chelate group to the agarose ensures true one-step purification.

Caratteristiche e vantaggi

  • High selectivity for higher purity
  • Unique non-charged hydrophilic linkage reduced non-specific binding
  • Binding Capacity for histidine-tagged protein (His-tag protein) is greater than 15 mg/mL
  • Highly cross-linked agarose matrix allows for purification under higher pressures
  • Binding under denaturing or non-denaturing conditions
  • One-step purification

Stato fisico

1:1 suspension in a 30% ethanol solution

Nota sulla preparazione

Ethanol must be removed prior to use. Wash affinity gel in deionized water to remove the ethanol, then equilibrate with equilibration buffer that is provided.

Note legali

FPLC is a trademark of Cytiva
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

Avvertenze

Danger

Classi di pericolo

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1A Inhalation - Flam. Liq. 3 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 - STOT SE 2

Organi bersaglio

Eyes,Central nervous system, Respiratory Tract

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

88.0 °F

Punto d’infiammabilità (°C)

31.1 °C

Dispositivi di protezione individuale

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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