G6137

Glutathione Peroxidase from bovine erythrocytes

lyophilized powder, ≥300 units/mg protein

• Sinonimo/i: GSH-Px, Glutathione:hydrogen-peroxide oxido-reductase
• Numero CAS: 9013-66-5
• Classificazione EC (Enzyme Commission): 1.11.1.9 (BRENDA, IUBMB)
• Numero CE: 232-749-6
• Numero MDL: MFCD00131195
• Codice UNSPSC: 12352204
• NACRES: NA.54

Proprietà

• Origine biologica: bovine erythrocytes
• Livello qualitativo: 200
• Stato: lyophilized powder
• Attività specifica: ≥300 units/mg protein
• PM: 84.5 kDa
• Composizione: Protein, 10-30% modified Warburg-Christian
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Glutathione peroxidase is an antioxidant enzyme that contains selenium.[1] It is present in the glandular epithelium of human endometrium.[2]

Applicazioni

Glutathione Peroxidase from bovine erythrocytes has been used as an oxygen radical scavenger to study its effect on cytotoxicity of 1,3-dilinoleoylglycerol (DLG) against E1A-3Y1 cells.[3]

Glutathione peroxidase from bovine erythrocytes was used as a positive control in cloning and characterization of full-length cDNAs encoding two glutathione peroxidases (GpXs) from Globodera rostochiensis.[4] It was used for the determination of glutathione peroxidase activity in human milk.[5]

Azioni biochim/fisiol

Glutathione peroxidase helps to reduce (peroxides) H₂O₂ to water and lipid peroxides to lipid alcohols.[1]

Glutathione peroxidase is an enzyme which reduced lipid hydroperoxides into their corresponding alcohols. It also reduces free hydrogen peroxide in to water. In vivo it is responsible for protecting hemoglobin from oxidative breakdown[6][7].

Protects cells against oxidative damage by catalyzing the reduction of hydrogen peroxide in the presence of reducing agent glutathione. In cellular membranes it may induce lipid peroxidation through the reduction of hydrogen peroxide or polyunsaturated fatty acid hydroperoxides.

Definizione di unità

One unit will catalyze the oxidation by H₂O₂ of 1.0 μmole of reduced glutathione to oxidized glutathione per min at pH 7.0 at 25 °C.

Stato fisico

Lyophilized powder containing 25% sucrose and 2.5% dithiothreitol with sodium phosphate buffer salts

Altre note

Note: At the reported pH optimum of 8.8, we have found the activity to be approx. 10 times that at pH 7.0. However, to remain consistent with literature and avoid complications arising from non-enzymatic oxidation of GSH, our unit is defined at pH 7.0.

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H334
• Consigli di prudenza: P261 - P284 - P501
• Classi di pericolo: Resp. Sens. 1
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)