FLAGIPT1
FLAG® Immunoprecipitation Kit
Sinonimo/i:
Anti-ddddk, Anti-dykddddk
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About This Item
Codice UNSPSC:
12352200
NACRES:
NA.32
Prodotti consigliati
impiego
kit sufficient for 50 reactions
Livello qualitativo
Classi chimiche degli analiti
proteins (FLAG® Affinity Gels)
tecniche
immunoprecipitation (IP): suitable
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
Descrizione generale
Sigma′s FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein. The immunoprecipitation is performed with the ANTI-FLAG M2 affinity gel, which is a highly specific monoclonal antibody covalently attached to agarose resin. The use of affinity resin allows an efficient binding of FLAG-tagged proteins without the need for preliminary steps and calibrations. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin with acidic conditions or by competition with FLAG peptide. The immunoprecipitated proteins can be detected for their size, post-translational modifications, and interactions on gel electrophoresis, and by activity assays.
Applicazioni
Learn more product details in our FLAG® application portal.
Caratteristiche e vantaggi
- Utilizes highly specific ANTI-FLAG M2 affinity gel
- No preliminary steps or calibrations
- 3X FLAG™ peptide provides efficient and gentle elution through direct competition
Altre note
All prodedures to be performed at 2-8°C.
Note legali
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Aquatic Chronic 3 - Eye Irrit. 2
Codice della classe di stoccaggio
10 - Combustible liquids
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