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46950

Sigma-Aldrich

Fluorescein 5(6)-isothiocyanate

BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)

Sinonimo/i:

FITC, Fluorescein isothiocyanate

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About This Item

Formula empirica (notazione di Hill):
C21H11NO5S
Numero CAS:
27072-45-3
Peso molecolare:
389.38
Beilstein:
1407295
Numero CE:
248-207-7
Numero MDL:
MFCD00005063
Codice UNSPSC:
12171500
ID PubChem:
329757273
NACRES:
NA.32

Livello qualitativo

100

Nome Commerciale

BioReagent

Saggio

≥90% (HPLC)

Fluorescenza

λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0

Compatibilità

suitable for fluorescence

Temperatura di conservazione

−20°C

Stringa SMILE

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1

InChI

1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
MHMNJMPURVTYEJ-UHFFFAOYSA-N

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Categorie correlate

Descrizione generale

Fluorescein 5(6)-isothiocyanate, also known as FITC, is a weakly acidic hydroxyxanthene whose overall size is small, with both carboxylic acid and isothiocyanate substituents on the pendant phenyl ring. The dye has a moderately sized, nonplanar aromatic system. The isothiocyanate group reacts with nucleophilic substituents (e.g. amino, hydroxyl, thiol) of biomolecules to be attached as a fluorescent label. The maximum emission of the dye is at 519nm in the same solvent.

Applicazioni

Fluorescein 5(6)-isothiocyanate (FITC) labelled molecules are used in non-microscopic immunoassays and physiological tracing experiments. FITC is widely used in flow cytometry experiments for staining proteins, nuclei, and subnuclear particles. White blood cells (WBCs) are marked with FITC to study their response toward a chemoattractant, eventually being measured.
For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography

Confezionamento

Bottomless glass bottle. Contents are inside inserted fused cone.

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Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificati d'analisi (COA)

Lot/Batch Number
BCCL3464
BCCL7007
BCCH4149
BCCJ7341
BCCG6116

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