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46955

Sigma-Aldrich

Fluorescein

for fluorescence, free acid

Sinonimo/i:

Acid Yellow 73

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250 MG
30,20 €
1 G
120,00 €
100 G
214,00 €
500 G
380,00 €

30,20 €


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Cambia visualizzazione
250 MG
30,20 €
1 G
120,00 €
100 G
214,00 €
500 G
380,00 €

About This Item

Formula empirica (notazione di Hill):
C20H12O5
Numero CAS:
Peso molecolare:
332.31
Colour Index:
45350
Beilstein:
94324
Numero CE:
Numero MDL:
Codice UNSPSC:
12171500
ID PubChem:
NACRES:
NA.32

30,20 €


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Grado

for fluorescence

Livello qualitativo

tecniche

titration: suitable

Fluorescenza

λex 490 nm; λem 514 nm in 0.1 M Tris pH 8.0

Compatibilità

suitable for fluorescence

Stringa SMILE

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5ccccc45)c1

InChI

1S/C20H12O5/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20/h1-10,21-22H
GNBHRKFJIUUOQI-UHFFFAOYSA-N

Informazioni sul gene

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Descrizione generale

Fluorescein dye is the most commonly used fluorescent probe.[1] It is used to stain epithelial defects and analyse the integrity of corneal epithelium. Fluorescein is used in the diagnosis of corneal perforation.[2] Oral or intravenous fluorescein is used in fluorescein angiography to detect diabetic retinopathy and choroidal neovascular membrane.[3]

Applicazioni

Fluorescein has been used:
  • in the permeability assay of Caco-2 cells[4][5]
  • to test its suitability to serve as lifetime fluorescence standards[6]
  • as a fluorophore for the fast recovery of fluorescence peaks and fluorophore concentration in the presence of hemoglobin[7]
  • as a fluorescent probe in oxygen radical absorbance capacity (ORAC) assay[8]

Pittogrammi

Exclamation mark

Avvertenze

Warning

Indicazioni di pericolo

Classi di pericolo

Eye Irrit. 2

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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Experimental recovery of intrinsic fluorescence and fluorophore concentration in the presence of hemoglobin: spectral effect of scattering and absorption on fluorescence
Du Le VN, et al.
Journal of Biomedical Optics, 20(12), 127003-127003 (2015)
Ocular health assessment
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Clinical Pediatric Nephrology, Second Edition, 221-318 (2007)
Absorption and fluorescence properties of fluorescein
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Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, 51(6), L7-L21 (1995)

Articoli

Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.

Fluorescence quenching microscopy visualizes 2D materials like graphene and MoS2 rapidly, inexpensively, and with high fidelity.

Domande

1–7 di 7 domande  
  1. What is a free acid?

    1 risposta
    1. A free acid is the acidic form of a substance in which all acidic groups are fully protonated.

      Utile?

  2. What is the size in kDa of this Fluorescein?

    1 risposta
    1. This product has a molecular weight size of 332.32 g/mol or 332.32 daltons. The kDa unit designates kilodaltons, 1 kDa is equivalent to 1000 daltons.

      Utile?

  3. How long can this product be kept once it's made into a solution? If it can be kept more than 1 day; what are the storage conditions? Thank you

    1 risposta
    1. The stability of this product is not determined. However, various sources state that this product is stable in solvent at -80°C for 6 months; at -20°C for 1 month (protect from light).

      Utile?

  4. What is the dye content of Product 46955, Fluorescein?

    1 risposta
    1. According to our supplier, the dye content of pure Fluorescein is a minimum of 90%. We do not test the purity of this product since it is not one of our specifications.

      Utile?

  5. How should Product 46955, Fluorescein, be stored?

    1 risposta
    1. We recommend storing this product at room temperature.

      Utile?

  6. What is the Department of Transportation shipping information for this product?

    1 risposta
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Utile?

  7. What is Product 46955, Fluorescein, soluble in?

    1 risposta
    1. According to the chemicals encyclopedia published by the Royal Society of Chemistry : 13th Edition, Fluorescein is soluble in hot alcohol and glacial acetic acid. Also, it is soluble in alkali hydroxides or carbonates. This product is insoluble in water, benzene, chloroform and ether.

      Utile?

Recensioni

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