FITC is not membrane permeable in healthy cells, it binds to surface proteins. Conjugates such as FITC-Dextran is an example of a more cell-permeable option.
F7250
Fluorescein isothiocyanate isomer I
≥90% purity (HPLC), powder
Sinonimo/i:
FITC, Fluorescein 5-isothiocyanate
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50 MG
38,20 €
100 MG
60,10 €
250 MG
109,00 €
500 MG
202,00 €
1 G
337,00 €
5 G
1.330,00 €
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Cambia visualizzazione
50 MG
38,20 €
100 MG
60,10 €
250 MG
109,00 €
500 MG
202,00 €
1 G
337,00 €
5 G
1.330,00 €
About This Item
Formula empirica (notazione di Hill):
C21H11NO5S
Numero CAS:
Peso molecolare:
389.38
Beilstein:
1407295
Numero CE:
Numero MDL:
Codice UNSPSC:
12171500
ID PubChem:
NACRES:
NA.47
Prodotti consigliati
Nome del prodotto
Fluorescein isothiocyanate isomer I, suitable for protein labeling, ≥90% (HPLC), powder
Saggio
≥90% (HPLC)
Stato
powder
tecniche
titration: suitable
Colore
orange to dark orange
Punto di fusione
>360 °C (lit.)
Solubilità
acetone: 1 mg/mL
Fluorescenza
λex 492 nm; λem 518 nm (green)
Compatibilità
suitable for protein labeling
applicazioni
diagnostic assay manufacturing
hematology
histology
Temperatura di conservazione
2-8°C
Stringa SMILE
Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
InChI
1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
MHMNJMPURVTYEJ-UHFFFAOYSA-N
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Categorie correlate
1 of 4
Questo articolo | F4274 | 34321-M | F3651 |
|---|---|---|---|
| technique(s) titration: suitable | technique(s) titration: suitable | technique(s) - | technique(s) titration: suitable |
| color orange to dark orange | color yellow to very dark yellow | color orange-red | color yellow to orange |
| fluorescence λex 492 nm; λem 518 nm (green) | fluorescence λex 492 nm; λem 518 nm (green) | fluorescence - | fluorescence - |
| form powder | form powder | form solid | form powder |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - | application(s) diagnostic assay manufacturing |
Descrizione generale
Fluorescein isothiocyanate (FITC) is yellow-orange in color with an absorption maximum at 495nm. Upon excitation, it emits a yellow-green color with an emission maximum at 525nm.
It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.[1][2]
Fluorescein isothiocyanate isomer I has been proposed as a contact sensitizer.[3]
It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.[1][2]
Fluorescein isothiocyanate isomer I has been proposed as a contact sensitizer.[3]
Applicazioni
Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)
Reagent for the FITC labeling of proteins; microsequencing of proteins and peptides (HPLC)
Biological applications include use as a fluorescent labeling reagent for proteins[4][5][6], a fluorescent reagent for protein tracing, and a reagent in the fluorescent antibody technique for the rapid identification of pathogens. It may be employed as the derivatization reagent for amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine and P-phenylethylamine in human urine during their capillary electrophoretic (CE) determination. It may be used for the preparation of fluorescent antibodies. It was employed for in vitro sensitization studies.
Biological applications include use as a fluorescent labeling reagent for proteins[4][5][6], a fluorescent reagent for protein tracing, and a reagent in the fluorescent antibody technique for the rapid identification of pathogens. It may be employed as the derivatization reagent for amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine and P-phenylethylamine in human urine during their capillary electrophoretic (CE) determination. It may be used for the preparation of fluorescent antibodies. It was employed for in vitro sensitization studies.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Resp. Sens. 1 - Skin Sens. 1
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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I clienti hanno visto anche
A Ramseier et al.
Electrophoresis, 19(16-17), 2967-2975 (1998-12-31)
Amines can easily be derivatized with fluorescein isothiocyanate isomer I (FITC) and analyzed by capillary electrophoresis (CE) using alkaline buffers with or without dodecyl sulfate micelles. This paper reports the CE analysis of FITC-derivatized amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine and beta-phenylethylamine in
Test of a two-pathway model for small-solute exchange across the capillary wall.
Fu BM
The American Journal of Physiology, 274, H2062-H2062 (1998)
S E Macatonia et al.
Immunology, 59(4), 509-514 (1986-12-01)
Lymph node cells taken 24 hr after skin-painting mice with the contact sensitizer fluorescein isothiocyanate (FITC) induce delayed-type hypersensitivity in recipient mice. Skin-painting increased the number of dendritic cells (DC) in the draining lymph nodes without significantly changing the number
Chromatographically pure fluorescein and tetramethylrhodamine isothiocyanates.
L C FELTON et al.
Analytical biochemistry, 2, 178-180 (1961-04-01)
Human monoclonal autoantibodies specific for the bullous pemphigoid antigen 1 (BPAg 1).
Peyron E
Journal of Immunology, 153, 1333-1333 (1994)
Articoli
Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.
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Are cell membranes of living cells permeable for FITC?
1 risposta-
Utile?
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Is the intensity of FITC (e.g., Product No. F7250) pH dependent?
1 risposta-
Yes; many articles have reported the pH dependence of the fluorescence of fluorescein derivatives. See the following references: Archives Biochem. Biophys., 133, 263 (1969); J. Immunol., 95, 1165 (1966); Archives Biochem. Biophys., 73, 1 (1958); Standard test for labeling efficiency of FITC with BSA: NCCLS Approved Standard: ASM-1, 2nd ed., June, 1975; Characterization of FITC-dyes by IR and TLC: Analytical Biochem., 29, 526 (1969); Fluorescent labeling of proteins: Proc. Nat. Acad. Sci., 72, 459 (1975); Amer. J. Pathol., 34, 1081 (1958); Fluorescent labeling of a lectin: Meth. Enzymol., 81, 65 (1982).
Utile?
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What is the Department of Transportation shipping information for this product?
1 risposta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Utile?
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After the FITC (e.g., Product No. F7250) is conjugated to the protein, what is the solution stability of the conjugated protein?
1 risposta-
The stability of the conjugated protein will be essentially the stability of the unconjugated protein. Some conjugated proteins might be stable when stored at -20°C and other conjugated proteins will have better solution stability at 2-8°C If the protein concentration is too low, this also has a negative effect upon the stability of the conjugated protein.
Utile?
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Is Product No. F7250, Fluorescein isothiocyanate isomer I, pharmaceutical grade?
1 risposta-
No. The product is considered suitable for research use only.
Utile?
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How stable is FITC Isomer 1 (e.g., Product No. F7250) once conjugated to an antibody?
1 risposta-
FITC can photo bleach fairly rapidly. The prepared solution should be stored in a dark container and protected from light. When viewing the antibody staining under a microscope, it is advisable to use subdued fluorescent light when possible. If the fluorescent light stays at one position for too long, photo bleaching is noticeable.
Utile?
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