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30911

Sigma-Aldrich

Denaturation solution for the isolation of RNA

BioUltra, for molecular biology

Sinonimo/i:

RNA isolation, denaturation

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About This Item

Numero MDL:
MFCD00217302
Codice UNSPSC:
12352202
NACRES:
NA.25

Grado

for molecular biology

Livello qualitativo

100

Nome Commerciale

BioUltra

Composizione

guanidine thiocyanate, 4 M
Na-citrate, 25 mM
sarcosyl, 0.5%

Impurezze

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

Indice di rifrazione

n20/D 1.433

pH

5.5-7.0

Anioni in tracce

chloride (Cl-): ≤500 mg/kg
sulfate (SO42-): ≤50 mg/kg

Cationi in tracce

Al: ≤2 mg/kg
As: ≤0.1 mg/kg
Ba: ≤2 mg/kg
Bi: ≤2 mg/kg
Ca: ≤10 mg/kg
Cd: ≤2 mg/kg
Co: ≤2 mg/kg
Cr: ≤2 mg/kg
Cu: ≤2 mg/kg
Fe: ≤2 mg/kg
K: ≤100 mg/kg
Li: ≤2 mg/kg
Mg: ≤2 mg/kg
Mn: ≤2 mg/kg
Mo: ≤2 mg/kg
Ni: ≤2 mg/kg
Pb: ≤2 mg/kg
Sr: ≤2 mg/kg
Zn: ≤2 mg/kg

λ

neat

Assorbanza UV

λ: 280 nm Amax: 1

Applicazioni

Denaturation solution for the isolation of RNA is a guanidine thiocyanate, sarcosyl based protein denaturization solution useful for the purification of mRNA.

Altre note

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

Pittogrammi

CorrosionExclamation mark
GHS05,GHS07

Avvertenze

Danger

Indicazioni di pericolo

H302 + H332,H314,H412

Classi di pericolo

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

Rischi supp

EUH032

Codice della classe di stoccaggio

8A - Combustible corrosive hazardous materials

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
BCCF1640
BCBS3081V
BCBK2156V
BCBH0592V
BCBG7029V

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Y L Tsai et al.
Applied and environmental microbiology, 57(3), 765-768 (1991-03-01)
A protocol for direct extraction of mRNA from soil samples was developed. Soil samples (10 g) were washed twice with 120 mM phosphate buffer (pH 5.2). The lysis of cells, fixation of RNA, and hydrolysis of DNA were achieved by
P Chomczynski et al.
Analytical biochemistry, 162(1), 156-159 (1987-04-01)
A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is

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