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Merck
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Documenti fondamentali

126575

Sigma-Aldrich

Albumin, Bovine Serum, Fraction V, Fatty Acid-Free

Sinonimo/i:

Albumin, Bovine Serum, Fraction V, Fatty Acid-Free

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10 G
150,00 €
100 G
717,00 €

150,00 €

Spedizione prevista il28 febbraio 2025Dettagli

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10 G
150,00 €
100 G
717,00 €

About This Item

Numero CAS:
9048-46-8
Numero MDL:
MFCD00797675
Codice UNSPSC:
12352202
NACRES:
NA.25

150,00 €

Spedizione prevista il28 febbraio 2025Dettagli

Richiedi un ordine bulk

Saggio

≥95% (cellulose acetate electrophoresis)

Stato

powder

Produttore/marchio commerciale

Calbiochem®

Condizioni di stoccaggio

OK to freeze

Impurezze

≤0.02% fatty acids
≤3% ash
≤5% moisture

Colore

white to light yellow
light yellow to light brown

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

Descrizione generale

Albumin, Bovine Serum, (BSA) Fraction V, Fatty Acid-Free is useful for immunodiagnostic assays. It is designed for use in serological testing, radioimmunoassay, and hormone response studies. It is also applicable in binding and transport studies. BSA is prepared by cold ethanol extraction (Cohn method) with a pH: 6.8-7.4.
Bovine serum albumin (BSA) is a non-glycosylated globular, α-helical protein belonging to the serum albumin family.[1][2] It consists of three domains with two sub-domains each and has 17-disulfide bonds.[1][2]

Applicazioni

Albumin, Bovine Serum, Fraction V, Fatty Acid-Free has been used:
  • as a component of: blocking buffer immunoelectron microscopy (IEM) for various cells[3]
  • mitochondrial isolation buffer (MIB) and mitochondria assay solution (MAS) to trap free fatty acids released during adipose tissue homogenization[4]
  • blocking buffer for wholemount staining of the esophageal epithelium (EE) in immunofluorescence[5]

Azioni biochim/fisiol

Bovine Serum Albumin (BSA), the most vital component of cell culture media, aids in embryonic stem cells (hESC) differentiation.[6] It aids the transportation of drugs, hormones, and fatty acids.[1] BSA is also used as a blocking agent in enzyme-linked immunosorbent assay (ELISA).[7]

Attenzione

Toxicity: Standard Handling (A)

Nota sulla preparazione

May take up to 24 h to fully dissolve.

Ricostituzione

Following reconstitution, store in the refrigerator (4°C). 0.1% NaN₃ may be added as a preservative. Stock solutions are stable for up to 3 months at 4°C.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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M Prats
European journal of biochemistry, 75(2), 619-625 (1977-05-16)
The L-(+)-Lactate:cytochrome c oxidoreductase or cytochrome b2 from the yeasts Saccharomyces cerevisiae and Hansenula anomala were partially hydrolysed in various concentrations of trypsin. Conditions were found which allowed the isolation from the Hansenula enzyme of a 140 000 +/- 10
Andrew W Ferree et al.
Autophagy, 9(11), 1887-1896 (2013-10-24)
To study mitochondrial protein age dynamics, we targeted a time-sensitive fluorescent protein, MitoTimer, to the mitochondrial matrix. Mitochondrial age was revealed by the integrated portions of young (green) and old (red) MitoTimer protein. Mitochondrial protein age was dependent on turnover
E Sandra Chocron et al.
Molecular endocrinology (Baltimore, Md.), 26(7), 1117-1128 (2012-05-10)
We previously demonstrated that the thyroid hormone, T(3), acutely stimulates mitochondrial metabolism in a thyroid hormone receptor (TR)-dependent manner. T(3) has also recently been shown to stimulate mitochondrial fatty acid oxidation (FAO). Here we report that TR-dependent stimulation of metabolism
Jacob M Serfass et al.
The Journal of biological chemistry, 292(24), 10097-10111 (2017-04-30)
Endocytosis, and the subsequent trafficking of endosomes, requires dynamic physical alterations in membrane shape that are mediated in part by endophilin proteins. The endophilin B family of proteins contains an N-terminal Bin/amphiphysin/Rvs (N-BAR) domain that induces membrane curvature to regulate
Soyeon Park et al.
Experimental & molecular medicine, 50(8), 1-14 (2018-08-05)
Increased fatty acid (FA) is often observed in highly proliferative tumors. FAs have been shown to modulate the secretion of proteins from tumor cells, contributing to tumor survival. However, the secreted factors affected by FA have not been systematically explored.
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