G005482

LC/MS Analysis of Lactic Acid Enantiomers in Urine on Astec^® CHIROBIOTIC^® R

application for HPLC

Tulajdonságok

• technika/technikák: HPLC: suitable
• tesztelési paraméterek: column: Astec CHIROBIOTIC R, 15 cm x 2.1 mm I.D., 5 μm particles (13019AST); mobile phase: [A] 33.3 mM ammonium acetate; [B] acetonitrile; (15:85, A:B); flow rate: 0.7 mL/min; column temp.: 4 °C; detector: (-)ESI; injection: 20 μL; sample: Urine extracts in 5% (v/v) acetic acid, 10% (v/v) H₂O and 85% (v/v) acetonitrile
• alkalmasság: application for HPLC
• alkalmazás(ok): clinical

Leírás

Analízis megjegyzés

Chromatogram supplied courtesy of H. Henry and associates at the Clinical Chemistry Laboratory, Centre Hospitalier Universitaire Vaudois, University of Lausanne, CH-1011, Switzerland. View the related full-length contributed article in Reporter 30.1.

Egyéb megjegyzések

Reference: Biomed. Chromatogr. 2011 "Sensitive determination of D-lactic acid and L-lactic acid in urine by high-performance liquid chromatography–tandem mass spectrometry" H. Henry, N. Marmy Conus, P. Steenhout, A. Béguin and O. Boulat, Published online in Wiley Online Library, wileyonlinelibrary.com/journal/bmc.

Jogi információk

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany

CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

Analit

L-(+)-Lactic acid, analytical standard
D-(−)-Lactic acid, ≥90%