567520-U
Discovery® HS F5 (5 µm) HPLC Columns
L × I.D. 25 cm × 10 mm, HPLC Column
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
41115700
eCl@ss:
32110501
NACRES:
SB.52
Javasolt termékek
Terméknév
Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 10 mm
Anyagok
stainless steel column
Minőségi szint
Ügynökség
suitable for USP L43
termékcsalád
Discovery®
Jellemzők
endcapped
gyártó/kereskedő neve
Discovery®
kiszerelés
1 ea of
a címkézés mértéke
12% Carbon loading
Paraméterek
≤70 °C temp. range
400 bar pressure (5801 psi)
technika/technikák
HPLC: suitable
H × belső átmérő
25 cm × 10 mm
külső felület
300 m2/g
felület bevonatolás
4 μmol/m2
szennyeződések
<10 ppm metals
Mátrix
silica gel, high purity, spherical particle platform
fully porous particle
mátrix aktív csoport
PFP (pentafluorophenyl) phase
részecskeméret
5 μm
pórusméret
120 Å
Üzemi pH tartomány
2-8
alkalmazás(ok)
food and beverages
elválasztástechnika
reversed phase
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Általános leírás
Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.
Tulajdonságok és előnyök
- Unique selectivity
- Similar retention to C18 (sometimes requires stronger mobile phase)
- Excellent peak shape
- Stable, low-bleed LC-MS separations
- Scalable separations from 3 to 10μm particle sizes
Jogi információk
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
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