T8443

Trizma^® Pre-set crystals

BioPerformance Certified, pH 8.0, average M_w 141.8

• Szinonimák: Tris crystals
• UNSPSC kód: 12161700
• NACRES: NA.25

Tulajdonságok

• grade: BioPerformance Certified
• Minőségi szint: 200
• form: crystalline powder
• molekulatömeg: average M_w 141.8
• technika/technikák: cell culture | mammalian: suitable
• szennyeződések: DNase, RNase, Protease, NICKase, none detected; endotoxin, tested; total aerobic microbial count, tested; ≤0.05 A₂₉₀; ≤0.5% water (Karl Fischer); ≤5 ppm Heavy metals (as Pb)
• pH: 8.00 (25 °C, 0.1 M)(lit.); 8.0
• hasznos pH-tartomány: 7.0-9.0
• oldhatóság: water: 0.66 g/mL, clear, colorless
• alkalmazás(ok): diagnostic assay manufacturing

Leírás

Alkalmazás

Dissolve in water to make a 0.1M solution. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.

Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.

If a different concentration is desired, note that there is a concentration effect on the pH of the solution.

The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases.

Egyéb megjegyzések

This product is a pre-mixed combination of Trizma base and Trizma HCl that can be used to prepare a common pH value for Tris buffer.

Certain pH electrodes do not give accurate results with Tris buffers.

Elkészítési megjegyzés

Trizma solutions can be autoclaved.

Jogi információk

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: dust mask type N95 (US), Eyeshields, Gloves