Ugrás a tartalomra
Merck

T2780

Sigma-Aldrich

Monoclonal Anti-Tropomyosin antibody produced in mouse

clone TM311, ascites fluid

Szinonimák:

Anti-Tropomyosin Antibody

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

MDL-szám:
UNSPSC kód:
12352203
NACRES:
NA.41

biológiai forrás

mouse

Minőségi szint

konjugátum

unconjugated

antitest forma

ascites fluid

antitest terméktípus

primary antibodies

klón

TM311, monoclonal

molekulatömeg

antigen 36-39 kDa

tartalmaz

15 mM sodium azide

faj reaktivitás

pig, chicken, bovine, rabbit, mouse, hamster, rat, human

technika/technikák

immunoprecipitation (IP): suitable
indirect ELISA: suitable
indirect immunofluorescence: 1:400 using chicken fibroblasts
microarray: suitable
western blot: suitable using human tissue and chicken gizzard extracts

izotípus

IgG1

UniProt elérési szám

kiszállítva

dry ice

tárolási hőmérséklet

−20°C

célzott transzláció utáni módosítás

unmodified

Géninformáció

bovine ... TPM1(281544)
chicken ... TPM1(396366)
human ... TPM1(7168)
mouse ... Tpm1(22003)
rat ... Tpm1(24851)

Általános leírás

Monoclonal Anti-Tropomyosin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Tropomyosin is a rigid rod-shaped protein closely associated with actin filaments. Non-muscle forms of tropomyosin have been identified in a wide range of cell types. Tropomyosin is made of two α helical polypeptide chains.

Immunogen

chicken gizzard tropomyosin.

Alkalmazás

Monoclonal Anti-Tropomyosin has been used:
  • in immunofluorescent labelling
  • in immunohistochemistry
  • in western blotting
  • in immunoblotting

Mouse monoclonal clone TM311 anti-Tropomyosin antibody is used to tag tropomyosin for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as immunoblotting, immunoprecipitation, and immunofluorescence. It is used as a probe to determine the presence and roles of tropomyosin in cytoskeletal structures.

Biokémiai/fiziológiai hatások

Tropomyosin together with troponin, regulate the binding of myosin to actin. Tropomyosin is a dimeric coiled-coil protein forming continuous polymers along the myosin-binding groove of actin. Various tropomyosin isoforms help to confer structure and function to actins in the cytoskeleton and in striated muscle function.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Tárolási osztály kódja

10 - Combustible liquids

WGK

nwg

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable


Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Ewelina Jurewicz et al.
Biochimica et biophysica acta, 1833(3), 761-766 (2012-12-26)
The CacyBP/SIP protein interacts with several targets, including actin. Since the majority of actin filaments are associated with tropomyosin, in this work we characterized binding of CacyBP/SIP to the actin-tropomyosin complex and examined the effects of CacyBP/SIP on actin filament
Tropomyosin-master regulator of actin filament function in the cytoskeleton
Gunning P W, et al.
Journal of Cell Science, 128(16), 2965-2974 (2015)
CENP-A is essential for cardiac progenitor cell proliferation
McGregor M, et al
Cell Cycle, 13(5), 739-748 (2014)
Activation of MAP kinases and phosphorylation of caldesmon in canine colonic smooth muscle.
Gerthoffer W T, et al.
The Journal of Physiology, 495(3), 597-609 (1996)
Iman Jalilian et al.
PloS one, 10(5), e0126214-e0126214 (2015-05-16)
The actin cytoskeleton is the primary polymer system within cells responsible for regulating cellular stiffness. While various actin binding proteins regulate the organization and dynamics of the actin cytoskeleton, the proteins responsible for regulating the mechanical properties of cells are

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