SAB4200400

Anti-WIPI-2 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Szinonimák: Anti-ATG18B, Anti-Atg21, Anti-CGI-50, Anti-WD repeat domain, phosphoinositide interacting 2, Anti-WIPI2
• UNSPSC kód: 12352203
• NACRES: NA.41
• konjugátum: unconjugated
• application: IP; WB
• klón: polyclonal
• faj reaktivitás: mouse, rat, human
• citations: 20
• technika/technikák: immunoprecipitation (IP): 2.5-5.0 μg using lysates of rat NRK cells.; western blot: 2-4 μg/mL using whole extracts of human G-361 and NIH-3T3 cells.

Tulajdonságok

• biológiai forrás: rabbit
• Minőségi szint: 200
• konjugátum: unconjugated
• antitest forma: affinity isolated antibody
• antitest terméktípus: primary antibodies
• klón: polyclonal
• Forma: buffered aqueous solution
• molekulatömeg: antigen ~49 kDa
• faj reaktivitás: mouse, rat, human
• koncentráció: ~1.0 mg/mL
• technika/technikák: immunoprecipitation (IP): 2.5-5.0 μg using lysates of rat NRK cells.; western blot: 2-4 μg/mL using whole extracts of human G-361 and NIH-3T3 cells.
• UniProt elérési szám: Q9Y4P8
• kiszállítva: dry ice
• tárolási hőmérséklet: −20°C
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: human ... WIPI2(26100) ; mouse ... Wipi2(74781) ; rat ... Wipi2(288498)

Leírás

Általános leírás

WIPI-2 belongs to the WIPI subfamily of WD40 repeat proteins. It is widely expressed in a variety of cell lines. The protein is characterized with a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids. It is a membrane constituent of autophagosomes and the plasma membrane.

Immunogén

synthetic peptide corresponding to the C-terminal region of human WIPI-2, conjugated to KLH. The corresponding sequence is identical in mouse and rat.

Alkalmazás

Anti-WIPI-2 (C-terminal) antibody produced in rabbit may be used in various immunochemical techniques including

• immunoblotting
• immunoprecipitation
• immunostaining.

Biokémiai/fiziológiai hatások

WIPI subfamily proteins acts as a key component of many essential biological functions such as signal transduction, transcription regulation and apoptosis. WIPI-2 is a phosphatidylinositol-3-phosphate binding protein that is needed for starvation induced autophagy. In addition, it is also essential for LC3 lipidation and it is linked to early autophagosomal structures together with Atg16L and ULK1.

Fizikai forma

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable