SAB4200468

Anti-JAM-A antibody, Mouse monoclonal

clone J10.4, purified from hybridoma cell culture

• Szinonimák: Monoclonal Anti-CD321, Monoclonal Anti-F11 receptor, Monoclonal Anti-F11R, Monoclonal Anti-JAM, Monoclonal Anti-JAM1, Monoclonal Anti-JAMA, Monoclonal Anti-JCAM, Monoclonal Anti-KAT, Monoclonal Anti-PAM-1
• UNSPSC kód: 12352203
• NACRES: NA.41
• konjugátum: unconjugated
• klón: J10.4, monoclonal
• faj reaktivitás: human
• citations: 9
• technika/technikák: immunoblotting: 1.0-2.0 μg/mL using HepG2 or A431 or A549 total cell extracts

Tulajdonságok

• biológiai forrás: mouse
• Minőségi szint: 200
• konjugátum: unconjugated
• antitest forma: purified immunoglobulin
• antitest terméktípus: primary antibodies
• klón: J10.4, monoclonal
• Forma: buffered aqueous solution
• molekulatömeg: antigen 37 kDa
• faj reaktivitás: human
• koncentráció: ~1.0 mg/mL
• technika/technikák: immunoblotting: 1.0-2.0 μg/mL using HepG2 or A431 or A549 total cell extracts
• izotípus: IgG1
• UniProt elérési szám: Q9Y624
• kiszállítva: dry ice
• tárolási hőmérséklet: −20°C
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: human ... F11R(50848)

Leírás

Általános leírás

Anti-JAM-A antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the hybridoma J10.4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant human JAM. Junctional adhesion molecule A (JAM-A) is expressed at tight junctions of endothelial and epithelial cells as well as on a variety of hematopoietic cells. It belongs to the transmembrane IgG glycoprotein family and is also expressed in monocytes, lymphocytes, neutrophils, dendritic cells, platelets, and spermatozoa. The JAM-A or F11R gene is mapped to human chromosome 1q23.3.

Egyediség

Anti-JAM-A antibody, Mouse monoclonal recognizes human JAM-A.

Immunogén

derived from the hybridoma J10.4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant human JAM fusion protein.

Alkalmazás

Anti-JAM-A antibody, Mouse monoclonal has been used in:

• western blotting
• staining for confocal microscopy
• immunoprecipitation
• immunocytochemistry
• flow cytometry

Biokémiai/fiziológiai hatások

Junctional adhesion molecule A(JAM-A) function has been linked with regulation of cell migration/invasion, platelet adhesion, cell polarity, cell proliferation, and epithelial or endothelial barrier function. However, the mechanisms by which JAM-A mediates these processes have not been well elucidated. JAM-A functional significance is connected with inflammation, angiogenesis, hypertension, ischemia/reperfusion, and atherosclerosis. JAM-A has also been linked to breast, endometrial, renal cell carcinomas and gastric cancer.

Fizikai forma

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Tárolás és stabilitás

For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 2
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable