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Sigma-Aldrich

ProteoPrep® Universal Extraction Kit

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
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About This Item

UNSPSC kód:
12352200
NACRES:
NA.32

form

dry powder

Minőségi szint

200

tárolási hőmérséklet

2-8°C

Related Categories

Alkalmazás

This kit features new and innovative detergents, and uses specially formulated reagents and an optimized protocol designed to generate two prepared subcellular fractions that are uniquely ready for two-dimensional (2D) electrophoresis.
  • Fraction 1: Soluble/Cytoplasmic Proteins
  • Fraction 2: Membrane Proteins
The special reducing and alkylating reagents produce samples that exhibit improved focusing and decreased streaking in 2D gels. The ProteoPrep Universal Extraction Kit was designed through a collaboration of Proteome Systems and Sigma research scientists.

Tulajdonságok és előnyök

  • Innovative detergent preparations - Highly improved solubility allows for higher protein loads and greater visibility of low abundance proteins on 2D gels.
  • Two pre-mixed solubilizaton solutions - Generates two distinct populations for easy 2D analysis.
  • Pre-measured reducing and alkylating reagents - Easy-to-use reagents provide improved IEF resolution.
  • Pre-weighed dry blends - Stable and easy to reconstitute
  • Conveniently Packaged - No waste. Use only the amount needed.

Jogi információk

ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

Product No.
Leírás
Biztonsági adatlap
  • C0356Protein Extraction Reagent Type 4 23 mLBiztonsági adatlap
  • T7567Tributylphosphine solution, 200 mM (in N-methyl-2-pyrrolidinone), liquid 5 x 0.5Biztonsági adatlap
  • A3221Iodoacetamide, Single use vial of 56 mg 5 x 56Biztonsági adatlap

Figyelmeztetés

Danger

Veszélyességi osztályok

Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3

Célzott szervek

Respiratory system

Tárolási osztály kódja

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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We present here a new approach that enabled the identification of a new protein from a bacterial strain with unknown genomic background using a combination of inverted PCR with degenerate primers derived from N-terminal protein sequences and high resolution peptide
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Journal of proteome research, 2(3), 303-311 (2003-06-20)
Abundant and hydrophilic nonmembrane proteins with isoelectric points below pH 8 are the predominant proteins identified in most proteomics projects. In yeast, however, low-abundance proteins make up 80% of the predicted proteome, approximately 50% have pl's above pH 8 and
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Declining cognitive performance is associated with increasing age, even in the absence of overt pathological processes. We and others have reported that declining cognitive performance is associated with age-related changes in brain glucose utilization, long-term potentiation and paired-pulse facilitation, protein
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