M9445

Matrix Metalloproteinase-2 from mouse

recombinant, expressed in NSO cells, >95% (SDS-PAGE), buffered aqueous glycerol solution

• Szinonimák: Collagenase Type IV 72 kDa, Gelatinase 72 kDa, Gelatinase A, MMP-2
• MDL-szám: MFCD01324322
• UNSPSC kód: 12352202
• NACRES: NA.32

Tulajdonságok

• rekombináns: expressed in NSO cells
• Minőségi szint: 200
• Teszt: >95% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• molekulatömeg: apparent mol wt ~72 kDa
• UniProt elérési szám: P33434; P33436
• kiszállítva: wet ice
• tárolási hőmérséklet: −20°C
• Géninformáció: mouse ... Mmp2(17390) ; rat ... Mmp2(81686)

Leírás

Általános leírás

Matrix Metalloproteinase-2 (MMP-2) also known as gelatinase or type IV collagenase is a 72kDa protein. MMP-2 is a member of matrix metalloproteinase (MMP) family of enzymes. Basic structure of MMP2 contains signal peptide domain that targets the enzyme for secretion, the pro-peptide domain, which is removed when the enzyme is activated and the catalytic site containing gelatin-binding domain.

Egyediség

The amino acid sequences 1-662 of the proenzymes of MMP-2 are identical between mouse and rat.

Alkalmazás

Matrix metalloproteinase-2 (MMP2) human has been used as a standard in zymography to measure proteolytic activity of MMP-2.

Biokémiai/fiziológiai hatások

Matrix Metalloproteinase-2 (MMP-2) cleaves gelatin, type IV, V, VII, X, and XI collagens, fibronectin, elastin, laminin, proteoglycans and a range of non extracellular matrix (ECM ) components. MMP-2 cleaves native type I collagen to N-terminal ¾ and C-terminal ¼ fragments identical to those generated by interstitial collagenases. MMP2 and MMP9 play an essential role in matrix degradation and they are implicated in the maintenance of neovascularization. In mice, deletion or inhibition of MMP2 protects against myocardial rupture.

MMP-2 degrades general matrix components and may have a role in processes such as host defense, cell proliferation, and protein turnover as well as tissue remodeling.

Fizikai forma

Supplied as a 0.2 μm filtered solution of 25 mM Tris, pH 7.5, 5 mM calcium chloride, 75 mM sodium chloride, 0.025% Brij^® 35 and 50% glycerol.

Analízis megjegyzés

The biological activity is measured by its ability to cleave a fluorogenic peptide sustrate.

Jogi információk

Brij is a registered trademark of Croda International PLC

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 2
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)