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Merck
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L5283

Sigma-Aldrich

Leukemia Inhibitory Factor human

≥95% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture

Szinonimák:

Human LIF, Human Leukemia Inhibitory Factor, hLIF, LIF, rLIF

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)

About This Item

MDL-szám:
MFCD00163950
UNSPSC kód:
12352202
NACRES:
NA.77

product name

Leukemia Inhibitory Factor human, LIF, recombinant, expressed in E. coli, 10 μg/ml, buffered aqueous solution (pH 7.4), suitable for cell culture

rekombináns

expressed in E. coli

Minőségi szint

200

Teszt

≥95% (SDS-PAGE)

form

buffered aqueous solution (pH 7.4)

hatékonyság

0.1-1.5 ng/mL ED50/EC50

minőség

endotoxin tested

technika/technikák

cell culture | mammalian: suitable

tárolási hőmérséklet

2-8°C

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Biokémiai/fiziológiai hatások

Leukemia Inhibitory Factor (LIF) is a pleiotropic glycoprotein originally described to inhibit the proliferation of the murine myeloid leukemic cell line M1, while inducing differentiation into macrophages. Other activities were later identified to LIF, which is known by a variety of synonyms, including DIF, D-factor, DIA, DRF, CNDF, HILDA, HSF-III, and MLPLI. Human LIF exerts its actions through a receptor comprising a 190 kDa LIF-binding α-chain (130 kDa, mouse) and a 130 kDa signal-transducing β-chain (gp130), which is shared with CNTF, OSM, L-6 and IL-11. As such, it is a member of the gp130 family of the cytokine receptor superfamily. LIF receptors have been identified on several cells, including monocytes, liver, placenta and embryonic stem cells. Natural LIF is heavily glycosylated, showing an apparent molecular weight of 32 kDa to 62 kDa, depending on the source, but absence of glycosylation appears not to affect its biological activity. A single gene encodes LIF, which is secreted as a single chain glycoprotein containing 180 amino acids for human or mouse with a conserved disulfide bond. Human and mouse LIF share 78% sequence homology. Human LIF can activate mouse cells, but mouse LIF cannot activate human cells.

Fizikai forma

Solution, 0.2 μm filtered, in phosphate buffered saline containing 0.02% TWEEN® 20.

Analízis megjegyzés

The proliferative activity of human LIF is measured in culture using the human leukemic cell line, TF-1.

Jogi információk

TWEEN is a registered trademark of Croda International PLC

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Gloves, type N95 (US)

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Dokumentumtár megtekintése

A Rodríguez et al.
Theriogenology, 67(5), 1092-1095 (2007-01-11)
Leukemia inhibitory factor (LIF) is a cytokine that shows conflicting effects on in vitro produced (IVP) bovine embryos. Bovine LIF (bLIF) has been cloned and used in culture, but there is no commercially available bLIF. Thus, researchers use human LIF
Arno Germond et al.
Analytical chemistry, 92(22), 14915-14923 (2020-10-29)
Monitoring cell-state transition in pluripotent cells is invaluable for application and basic research. In this study, we demonstrate the pertinence of noninvasive, label-free Raman spectroscopy to monitor and characterize the cell-state transition of mouse stem cells undergoing reprogramming. Using an
Pluripotent stem cell-derived models of neurological diseases reveal early transcriptional heterogeneity.
Sorek, et al.
Genome Biology, 22, 73-73 (2021)
Benjamin J Hale et al.
Reproductive biology and endocrinology : RB&E, 19(1), 107-107 (2021-07-11)
Heat stress (HS) occurs when body heat accumulation exceeds heat dissipation and is associated with swine seasonal infertility. HS contributes to compromised oocyte integrity and reduced embryo development. Autophagy is a potential mechanism for the oocyte to mitigate the detrimental
N M Gough et al.
Proceedings of the National Academy of Sciences of the United States of America, 85(8), 2623-2627 (1988-04-01)
A human homologue of the recently cloned murine leukemia-inhibitory factor (LIF) gene was isolated from a genomic library by using the murine cDNA as a hybridization probe. The nucleotide sequence of the human gene indicated that human LIF has 78%
View All Related Papers

Cikkek

Leukemia Inhibitory Factor (LIF) Protein

Leukemia Inhibitory Factor (LIF) is a stem cell growth factor used for the in vitro culture of pluripotent mouse embryonic stem cells (ES cells).

Naive Pluripotent Stem Cell Culture in Serum-Free Media

Naive pluripotent stem cells cultured in vitro using specialized media and inhibitors mimic "ground-state" cells from blastocysts.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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