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Merck
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I5283

Sigma-Aldrich

Anti-INCENP antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Szinonimák:

Anti-Inner Centromere Protein

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)

About This Item

MDL-szám:
MFCD05665235
UNSPSC kód:
12352203
NACRES:
NA.41

biológiai forrás

rabbit

Minőségi szint

200

konjugátum

unconjugated

antitest forma

IgG fraction of antiserum

antitest terméktípus

primary antibodies

klón

polyclonal

form

buffered aqueous solution

molekulatömeg

antigen ~106 kDa

faj reaktivitás

human, mouse, rat

technika/technikák

indirect immunofluorescence: 1:100 using HeLa cells
microarray: suitable
western blot (chemiluminescent): 1:2,000 using whole extract of mouse NIH3T3 and rat PC12 cells
western blot (chemiluminescent): 1:5,000 using nuclear extract of human HeLa cells

UniProt elérési szám

Q9NQS7

kiszállítva

dry ice

tárolási hőmérséklet

−20°C

célzott transzláció utáni módosítás

unmodified

Géninformáció

human ... INCENP(3619)
mouse ... Incenp(16319)
rat ... Incenp(293733)

Általános leírás

The gene INCENP (inner centromere protein) encodes an inner centromere protein that forms a component of the chromosomal passenger complex (CPC). It is found to be localized to inner centromeres during mitosis. The CPC also contains Aurora B, survivin, and Borealin.

Immunogen

synthetic peptide corresponding to amino acid residues 884-901 of human INCENP with N-terminal added cysteine conjugated to KLH. The corresponding sequence differs by one amino acid in chicken and Xenopus laevis and by two amino acids in mouse.

Alkalmazás

Anti-INCENP antibody produced in rabbit has been used in western blotting and immunofluorescence.

Biokémiai/fiziológiai hatások

Studies in knockout mice established INCENP (inner centromere protein) as essential for mouse development and viability. Loss of INCENP results in chromosome segregation defects and failures in cytokinesis.
The inner centromere protein encoded by the INCENP gene is phosphorylated by Cdk1 (cyclin dependent kinase 1) in mitosis. It is found to be associated with Plk1 (Polo-like kinase) in a polo-box–dependent manner. Plk1 is a serine/threonine kinase that functions in cell cycle, especially in mitosis. INCEP participates in the localization of Bub1 (Budding uninhibited by benzimidazole 1), a serine/threonine kinase involved in spindle checkpoint, to kinetochore. This, in turn, promotes the recruitment of Plk1 to kinetochores. Phosphorylated INCENP stimulates the activity of Aurora B kinase at centromeres and the central spindle that is important for chromosome segregation and cytokinesis, respectively.

Fizikai forma

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Tárolási osztály kódja

10 - Combustible liquids

WGK

WGK 2

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Dokumentumtár megtekintése

A Cul3-based E3 ligase removes Aurora B from mitotic chromosomes, regulating mitotic progression and completion of cytokinesis in human cells
Sumara I, et al.
Developmental Cell, 12(6), 887-900 (2007)
Lin-Ing Wang et al.
PLoS genetics, 15(5), e1008072-e1008072 (2019-06-01)
Sister centromere fusion is a process unique to meiosis that promotes co-orientation of the sister kinetochores, ensuring they attach to microtubules from the same pole during metaphase I. We have found that the kinetochore protein SPC105R/KNL1 and Protein Phosphatase 1
Reiko Honda et al.
Molecular biology of the cell, 14(8), 3325-3341 (2003-08-20)
The function of the Aurora B kinase at centromeres and the central spindle is crucial for chromosome segregation and cytokinesis, respectively. Herein, we have investigated the regulation of human Aurora B by its complex partners inner centromere protein (INCENP) and
Renee J Tamming et al.
Cell reports, 31(13), 107838-107838 (2020-07-02)
ATRX gene mutations have been identified in syndromic and non-syndromic intellectual disabilities in humans. ATRX is known to maintain genomic stability in neuroprogenitor cells, but its function in differentiated neurons and memory processes remains largely unresolved. Here, we show that
Fangwei Wang et al.
The Journal of cell biology, 199(2), 251-268 (2012-10-17)
Haspin phosphorylates histone H3 at threonine-3 (H3T3ph), providing a docking site for the Aurora B complex at centromeres. Aurora B functions to correct improper kinetochore-microtubule attachments and alert the spindle checkpoint to the presence of misaligned chromosomes. We show that
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