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Merck

I3909

Sigma-Aldrich

Ionomycin calcium salt

Ready Made Solution, from Streptomyces conglobatus, 1 mM in DMSO

Szinonimák:

Calcium Ionophore Ionomycin

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

Tapasztalati képlet (Hill-képlet):
C41H70CaO9
Molekulatömeg:
747.07
EC-szám:
MDL-szám:
UNSPSC kód:
51102829
PubChem Substance ID:
NACRES:
NA.85

biológiai forrás

Streptomyces conglobatus

Minőségi szint

Teszt

≥98% (HPLC)

eltarthatósági idő

2 yr

tárolási körülmény

(Tightly closed. )

koncentráció

1 mM in DMSO

szín

colorless

mp

18.4 °C

oldhatóság

DMSO: 1 mM

antibiotikus hatásspektrum

Gram-positive bacteria

Hatásmechanizmus

cell membrane | interferes

kiszállítva

wet ice

tárolási hőmérséklet

−20°C

SMILES string

C[C@@H]1CCC(=O)O[Ca]OC(=CC(=O)[C@@H](C)C[C@@H](C)C1)[C@H](C)C[C@H](C)C\C=C\[C@@H](C)[C@@H](O)[C@@H](C)[C@@H](O)C[C@@H]2CC[C@](C)(O2)[C@H]3CC[C@](C)(O3)[C@@H](C)O

InChI

1S/C41H72O9.Ca/c1-25(21-29(5)34(43)24-35(44)30(6)22-27(3)20-26(2)14-15-38(46)47)12-11-13-28(4)39(48)31(7)36(45)23-33-16-18-41(10,49-33)37-17-19-40(9,50-37)32(8)42;/h11,13,24-33,36-37,39,42-43,45,48H,12,14-23H2,1-10H3,(H,46,47);/q;+2/p-2/b13-11+,34-24-;/t25-,26-,27+,28-,29-,30+,31+,32-,33+,36+,37-,39-,40+,41+;/m1./s1

Nemzetközi kémiai azonosító kulcs

WKRWUYKLUMMAKG-WYGBAUISSA-L

Általános leírás

Ionomycin is a calcium ionophore commonly derived from produced by the bacterium Streptomyces conglobatus, which means that it helps to transport calcium ions across cell membranes. It is a powerful tool for studying calcium signaling and its role in various biological processes, including cell activation, secretion, proliferation, and apoptosis.

Ionomycin is commonly used in cell biology experiments to induce a rapid and controlled increase in intracellular calcium levels. This allows to study the downstream effects of calcium signaling pathways, such as the activation of enzymes, the release of hormones, and the changes in gene expression.Ionomycin is also used to investigate the role of calcium in specific cellular processes, such as cell death, immune cell activation, and muscle contraction. For example, ionomycin can be used to induce apoptosis in cancer cells, study the activation of T cells, and investigate the role of calcium in muscle contraction.

Alkalmazás

Ionomycin finds application in:
  • Study of calcium signaling and its role in various biological processes
  • Investigation of intracellular calcium levels, calcium-dependent enzymes, and cellular responses triggered by calcium influx
  • Induction of a rapid and controlled increase in intracellular calcium levels in cell biology experiments
  • Elucidation of specific cellular mechanisms and signaling pathways in combination with other substances, such as fluorescent calcium indicators or protein kinase activators
Ionomycin has been used:
  • to induce central demyelination
  • inhibit adrenal bovine TREK-1 channels
  • to regulate cell division of mature human B cells

Biokémiai/fiziológiai hatások

Ionomycin is an ionophoric antibiotic that binds Pb2+ and Ca2+, and serves as an effective mobile carrier of both cations.

Mode of Action: Facilitates the transfer of calcium ions (Ca2+) across biological membranes

Antimicrobial Spectrum: Active against Gram-Positive bacteria
Ionomycin is an ionophoric antibiotic which binds Pb2+ and Ca2+, and serves as an effective mobile carrier of both cations. Ionomycin is more effective than A23187 as a mobile ion carrier for Ca2+. It is non-fluorescent. It is used to study Ca2+ transport across biological membranes and induces apoptotic degeneration of embryonic cortical neurons.

Tulajdonságok és előnyök

  • Ready available solution reduce the need for preparation time
  • Efficient choice for studying calcium signaling and its role in various biological processes
  • Allows for rapid and controlled manipulation of intracellular calcium levels
  • Versatile and widely used in cell biology research

Egyéb megjegyzések

For additional information on our range of Biochemicals, please complete this form.

Tárolási osztály kódja

10 - Combustible liquids

WGK

WGK 1

Lobbanási pont (F)

188.6 °F - closed cup

Lobbanási pont (C)

87 °C - closed cup

Egyéni védőeszköz

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Analitikai tanúsítványok (COA)

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Dokumentumtár megtekintése

K M Kim et al.
Journal of immunology (Baltimore, Md. : 1950), 148(6), 1797-1803 (1992-03-15)
The growth of a human B lymphoma cell line B104, an experimental model for mature B cells, was inhibited by ionomycin but not 12-O-tetradecanoylphorbol-13-acetate (TPA). Ionomycin inhibited B104 cells from entering into the M phase of the cell cycle without
K J Smith et al.
Brain : a journal of neurology, 117 ( Pt 6), 1351-1356 (1994-12-01)
The effects of injecting the calcium-selective ionophore, ionomycin, into myelinated tracts in the dorsal columns of adult rat spinal cords were examined electron microscopically. In vivo, ionomycin induced a primary vesicular demyelination, together with a variable degree of axonal degeneration
Fabio Arturo Iannotti et al.
British journal of pharmacology, 176(10), 1568-1584 (2018-08-04)
Duchenne muscular dystrophy (DMD), caused by dystrophin deficiency, results in chronic inflammation and irreversible skeletal muscle degeneration. Moreover, the associated impairment of autophagy greatly contributes to the aggravation of muscle damage. We explored the possibility of using non-euphoric compounds present
Thorsten Schäfer et al.
Allergy, 65(10), 1242-1255 (2010-03-27)
Mast cells are secretory immune cells whose degranulation can provoke acute allergic reactions. It is presently unclear, however, whether an individual mast cell can repeatedly degranulate or turns dysfunctional after a single antigen stimulus. This work thus aims to better
John J Enyeart et al.
American journal of physiology. Cell physiology, 301(3), C619-C629 (2011-05-27)
Bovine adrenocortical cells express bTREK-1 K(+) (bovine KCNK2) channels that are inhibited by ANG II through a Gq-coupled receptor by separate Ca(2+) and ATP hydrolysis-dependent signaling pathways. Whole cell and single patch clamp recording from adrenal zona fasciculata (AZF) cells

Cikkek

This study presents an example of a typical ELISpot assay for quantifying the number of pre-existing, antigen-reactive T cells from peripheral blood mononuclear cell (PBMC) samples obtained from donors previously infected with cytomegalovirus (CMV).

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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