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GE28-9670-56

Protein A Mag Sepharose™ Xtra

Cytiva 28-9670-56, pack of 2 × 1 mL

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
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About This Item

UNSPSC kód:
41106500
NACRES:
NA.56

ligand

native protein A

kiszerelés

pack of 2 × 1 mL

gyártó/kereskedő neve

Cytiva 28-9670-56

tárolási körülmény

(20% Ethanol, 10% medium slurry)

parameter

Room temperature temp. range

matrix

paramagnetic, spherical, highly cross-linked agarose particles

kapacitás

27 mg binding capacity(human IgG/ml gel)

tárolási hőmérséklet

2-8°C

Általános leírás

protein A Mag Sepharose™ are magnetic beads designed to simplify small-scale purification and screening of antibodies. Based on Sepharose™ with native protein A as ligand.

Alkalmazás

Protein A Mag Sepharose™ Xtra magnetic beads are designed for rapid, small-scale purification and screening of monoclonal and polyclonal antibodies from serum and cell supernatants. The high capacity, > 27 mg human IgG/mL medium allow for the efficient capture of the antibody with high yield and purity. Approximately 350 μg purified human IgG can be obtained from each purification run.

Robust parallel screening of antibodies with high reproducibility.

Together with MagRack 6, a separation tool for handling the beads in microcentrifuge tubes, up to six samples can be processed in parallel. You can easily screen a larger number of samples in parallel with high throughput on a robotic device.

Tulajdonságok és előnyök

  • Visible and dense Sepharose™ based magnetic beads with Protein A ligand, easy to spot and collect bound target Protein
  • Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents
  • Simple capture of target Protein in small or large sample volumes (Low microliter to high milliliter scale)
  • Optimized high capacity for efficient small-scale purification and screening of antibodies from various species
  • High purity and yield

Tárolás és stabilitás

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analízis megjegyzés

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Jogi információk

Sepharose is a trademark of Cytiva

Piktogramok

Flame
GHS02

Figyelmeztetés

Warning

Figyelmeztető mondatok

H226

Óvintézkedésre vonatkozó mondatok

P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501

Tárolási osztály kódja

3 - Flammable liquids

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Lukas Leiendecker et al.
EMBO molecular medicine, 12(11), e12525-e12525 (2020-10-08)
Merkel cell carcinoma (MCC) is a highly aggressive, neuroendocrine skin cancer that lacks actionable mutations, which could be utilized for targeted therapies. Epigenetic regulators governing cell identity may represent unexplored therapeutic entry points. Here, we targeted epigenetic regulators in a
Qingzhou Ji et al.
Nucleic acids research, 42(10), 6158-6167 (2014-05-06)
Artificial transcription factors are powerful tools for regulating gene expression. Here we report results with engineered zinc-finger transcription factors (ZF-TFs) targeting four protein-coding genes, OCT4, SOX2, KLF4 and c-MYC, and one noncoding ribonucleic acid (RNA) gene, the microRNA (miRNA) miR302/367

Cikkek

Purification Using Protein A-based Chromatography Media

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

Immunoprecipitation Techniques

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Protocols

Purification of IgG Antibodies with Mag Sepharose®

Protein A Mag Sepharose® Xtra and Protein G Mag Sepharose® Xtra enable high-capacity small-scale antibody purification and screening.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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