GE17-5131-01

GSTrap^™ Fast Flow

Cytiva 17-5131-01, pack of 1 × 5 mL

• UNSPSC kód: 23151817
• NACRES: NA.25

Tulajdonságok

• ligand: glutathione and 10-carbon linker arm
• kiszerelés: pack of 1 × 5 mL
• gyártó/kereskedő neve: Cytiva 17-5131-01
• tárolási körülmény: (20% Ethanol)
• parameter: <20 mL/min flow rate; 42 psi
• ágyméret: 16 mm × 25 mm
• ágytérfogat: 5 mL
• oszlop belső átmérő: 16 mm
• Mátrix: highly cross-linked 4% agarose
• átlagos átmérő: 90 μm
• cleaning: 3-12
• Működési tartomány: 3-12
• kapacitás: >10 mg/mL binding capacity
• tárolási hőmérséklet: 2-8°C

Leírás

Általános leírás

GSTrap^™ FF columns are prepacked Glutathione Sepharose^™ Fast FLow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Alkalmazás

GST*-tagged Proteins can be purified directly from pretreated bacterial lysates using GSTrap^™ FF.GST-tagged Proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged Proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose^™ 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA^® design or FPLC^™ System.

Tulajdonságok és előnyök

• Fast and simple one-step purification of GST-tagged Proteins.
• Mild elution conditions preserving Protein antigenicity and function.
• Easy scale-up by connecting the columns in series.
• Sepharose^™ 4 Fast FLow provides good fLow properties.

Tárolás és stabilitás

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analízis megjegyzés

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Egyéb megjegyzések

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Jogi információk

FPLC is a trademark of Cytiva

GSTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

Biztonsági adatok

• Piktogramok: GHS02
• Figyelmeztetés: Warning
• Figyelmeztető mondatok: H226
• Óvintézkedésre vonatkozó mondatok: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Tárolási osztály kódja: 3 - Flammable liquids