Összes fotó(1)
Fontos dokumentumok
F4771
Monoclonal Anti-Fibroblast Surface Protein antibody produced in mouse
clone 1B10, ascites fluid
Szinonimák:
Fibroblast Surface Protein Antibody, Fibroblast Surface Protein Antibody - Monoclonal Anti-Fibroblast Surface Protein antibody produced in mouse
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
konjugátum:
unconjugated
application:
FACS
IHC (f)
complement-mediated cytotoxicity assay
IHC (f)
complement-mediated cytotoxicity assay
klón:
1B10, monoclonal
faj reaktivitás:
human
citations:
25
technika/technikák:
complement-mediated cytotoxicity assay: 1:500
flow cytometry: suitable
immunohistochemistry (frozen sections): suitable
flow cytometry: suitable
immunohistochemistry (frozen sections): suitable
Javasolt termékek
biológiai forrás
mouse
Minőségi szint
konjugátum
unconjugated
antitest forma
ascites fluid
antitest terméktípus
primary antibodies
klón
1B10, monoclonal
molekulatömeg
antigen 43-72-80 kDa
tartalmaz
15 mM sodium azide
faj reaktivitás
human
technika/technikák
complement-mediated cytotoxicity assay: 1:500
flow cytometry: suitable
immunohistochemistry (frozen sections): suitable
izotípus
IgM
kiszállítva
dry ice
tárolási hőmérséklet
−20°C
célzott transzláció utáni módosítás
unmodified
Általános leírás
Monoclonal Anti-Human Fibroblast Surface Protein (mouse IgM isotype) is derived from the 1B10 hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with cultured human thymic fibroblasts. Human fibroblast surface protein is a fibroblast antigen that is expressed mainly on human synovial, foreskin and thymic fibroblasts and is absent on human epithelial cells and lymphocytes.
Immunogén
cultured human thymic fibroblasts
Alkalmazás
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Monoclonal Anti-Fibroblast Surface Protein antibody produced in mouse has been used in:
- immunofluorescence
- flow cytometry
- immunohistochemistry
- immunoblotting
Monoclonal anti-fibroblast surface protein antibody can be used in flow cytometry of human fibroblasts and monocytes. It is also useful in immunoblotting and western blotting.
Biokémiai/fiziológiai hatások
Monoclonal Anti-Fibroblast Surface Protein antibody is also used to remove fibroblasts from human cultured cells by inhibiting fibroblasts adherence to the culture vessel or by cytotoxic effect in the presence of rabbit complement. In addition, apart from the functional significance of cells characteristics, the characterization of cell lines is important for correlation of cultures with tissue origin, identification of the lineage to which the cells belong or the precursor status of the cells. Monoclonal antibodies to fibroblast surface protein should provide a powerful tool for control of fibroblast growth in a variety of human cell culture systems, such as thymic epithelial (TE) cell cultures allowing the growth of highly enriched populations of the TE cells.
Cél megnevezése
This fibroblast antigen has been demonstrated on human synovial, mammary, foreskin and thymic fibroblasts and in malignant fibrosarcoma tissue. The antigen is both cell membrane and lysosome associated.
Analízis megjegyzés
Working dilution is at least 1:500 by complement-mediated microcytotoxicity assay.
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Nem találja a megfelelő terméket?
Próbálja ki a Termékválasztó eszköz. eszközt
javasolt
Tárolási osztály kódja
10 - Combustible liquids
WGK
nwg
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Válasszon a legfrissebb verziók közül:
Analitikai tanúsítványok (COA)
Lot/Batch Number
Nem találja a megfelelő verziót?
Ha egy adott verzióra van szüksége, a tétel- vagy cikkszám alapján rákereshet egy adott tanúsítványra.
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Az ügyfelek ezeket is megtekintették
Carcinoma-Associated Fibroblast-Like Differentiation of Human Mesenchymal Stem Cells
Mishra PJ, et al.
Cancer research, 68(11), 4331-4339 (2008)
M Olovsson et al.
Gynecologic and obstetric investigation, 49(3), 165-169 (2000-03-24)
To investigate the ability of human uterine myocytes to grow under anaerobic conditions for a prolonged time period. Cells were isolated from fundal myometrium and cultured until subconfluency. The cell type was confirmed by immunostaining for the smooth muscle cell-specific
Yuki Sekino et al.
FEBS open bio (2021-06-12)
There are no human cancer cell lines of external auditory canal origin available for research use. This report describes the establishment of a culture condition for external auditory canal squamous cell carcinoma, derived from human tumor tissue. Successive squamous cell
Qiyuan Zhou et al.
American journal of respiratory cell and molecular biology, 54(5), 728-739 (2015-10-22)
We have reported that von Hippel-Lindau protein (pVHL) expression is elevated in human and mouse fibrotic lungs and that overexpression of pVHL stimulates fibroblast proliferation. We sought to determine whether loss of pVHL in fibroblasts prevents injury and fibrosis in
Dana Simkova et al.
Oncotarget, 7(32), 52045-52060 (2016-07-14)
Asporin has been reported as a tumor suppressor in breast cancer, while asporin-activated invasion has been described in gastric cancer. According to our in silico search, high asporin expresion associates with significantly better relapse free survival (RFS) in patients with
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
Lépjen kapcsolatba a szaktanácsadással